Oogenesis of Lithodes santolla: histological and histochemical characterization

ABSTRACT Although Lithodes santolla is a resource with high commercial importance that has aroused interest in controlled reproduction in captivity, currently, little knowledge is available about basic reproductive aspects of this species. This research study describes detailed oogenesis stages at t...

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Autores principales:	Arcos-Ortega,Guadalupe F., Serrano-Silvas,Santos Rene, Rodríguez-Jaramillo,Carmen, Acuña-Gómez,Eliana Paola, Schofield-Astorga,Diana C., Olave-Solar,Carlos D.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Lithodes santolla oogenesis gonad maturity quantitative histology Magellan and Chilean Antarctic Region
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000300480
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spelling	oai:scielo:S0718-560X20190003004802019-08-09Oogenesis of Lithodes santolla: histological and histochemical characterizationArcos-Ortega,Guadalupe F.Serrano-Silvas,Santos ReneRodríguez-Jaramillo,CarmenAcuña-Gómez,Eliana PaolaSchofield-Astorga,Diana C.Olave-Solar,Carlos D. Lithodes santolla oogenesis gonad maturity quantitative histology Magellan and Chilean Antarctic Region ABSTRACT Although Lithodes santolla is a resource with high commercial importance that has aroused interest in controlled reproduction in captivity, currently, little knowledge is available about basic reproductive aspects of this species. This research study describes detailed oogenesis stages at the histological level in adult females of the southern king crab of the Magellan and Chilean Antarctic Region. Oogenesis was quantitatively analyzed from the proliferation of 10–24 μm oogonia and during oocyte growth. Five stages of ovary development were identified: multiplication (0), previtellogenesis (I), vitellogenesis (II), maturity (III) and spawning (IV). Furthermore, 11 oocyte substages were distinguished: oogonia, chromatin nucleolus, early perinucleolus, late perinucleolus, oil globule, early vitellin globule, late vitellin globule, postvitelogenic, germinal vesicle migration and germinal vesicle breakdown. Primary vitellogenesis occurred in oocytes 180–185 μm, containing acidophilic globules and surrounded by a layer of thick follicle cells (20-40 μm). Secondary vitellogenesis was evident in oocytes at 315-321 μm with numerous acidophilic granules in the cytoplasm and surrounded by thin follicle cells; then, cortical crypts appeared, indicating the prematuration stage and preparation for ovulation. This study allows establishing gonadic changes that occur during a reproductive cycle of female L. santolla and help to strengthen aquaculture initiatives and management in the Magellan and Chilean Antarctic Region.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.3 20192019-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000300480en10.3856/vol47-issue3-fulltext-10
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lithodes santolla oogenesis gonad maturity quantitative histology Magellan and Chilean Antarctic Region
spellingShingle	Lithodes santolla oogenesis gonad maturity quantitative histology Magellan and Chilean Antarctic Region Arcos-Ortega,Guadalupe F. Serrano-Silvas,Santos Rene Rodríguez-Jaramillo,Carmen Acuña-Gómez,Eliana Paola Schofield-Astorga,Diana C. Olave-Solar,Carlos D. Oogenesis of Lithodes santolla: histological and histochemical characterization
description	ABSTRACT Although Lithodes santolla is a resource with high commercial importance that has aroused interest in controlled reproduction in captivity, currently, little knowledge is available about basic reproductive aspects of this species. This research study describes detailed oogenesis stages at the histological level in adult females of the southern king crab of the Magellan and Chilean Antarctic Region. Oogenesis was quantitatively analyzed from the proliferation of 10–24 μm oogonia and during oocyte growth. Five stages of ovary development were identified: multiplication (0), previtellogenesis (I), vitellogenesis (II), maturity (III) and spawning (IV). Furthermore, 11 oocyte substages were distinguished: oogonia, chromatin nucleolus, early perinucleolus, late perinucleolus, oil globule, early vitellin globule, late vitellin globule, postvitelogenic, germinal vesicle migration and germinal vesicle breakdown. Primary vitellogenesis occurred in oocytes 180–185 μm, containing acidophilic globules and surrounded by a layer of thick follicle cells (20-40 μm). Secondary vitellogenesis was evident in oocytes at 315-321 μm with numerous acidophilic granules in the cytoplasm and surrounded by thin follicle cells; then, cortical crypts appeared, indicating the prematuration stage and preparation for ovulation. This study allows establishing gonadic changes that occur during a reproductive cycle of female L. santolla and help to strengthen aquaculture initiatives and management in the Magellan and Chilean Antarctic Region.
author	Arcos-Ortega,Guadalupe F. Serrano-Silvas,Santos Rene Rodríguez-Jaramillo,Carmen Acuña-Gómez,Eliana Paola Schofield-Astorga,Diana C. Olave-Solar,Carlos D.
author_facet	Arcos-Ortega,Guadalupe F. Serrano-Silvas,Santos Rene Rodríguez-Jaramillo,Carmen Acuña-Gómez,Eliana Paola Schofield-Astorga,Diana C. Olave-Solar,Carlos D.
author_sort	Arcos-Ortega,Guadalupe F.
title	Oogenesis of Lithodes santolla: histological and histochemical characterization
title_short	Oogenesis of Lithodes santolla: histological and histochemical characterization
title_full	Oogenesis of Lithodes santolla: histological and histochemical characterization
title_fullStr	Oogenesis of Lithodes santolla: histological and histochemical characterization
title_full_unstemmed	Oogenesis of Lithodes santolla: histological and histochemical characterization
title_sort	oogenesis of lithodes santolla: histological and histochemical characterization
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000300480
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_version_	1714205222506594304

Oogenesis of Lithodes santolla: histological and histochemical characterization

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