Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

ABSTRACT The effects of Gymnodynium catenatum and Prorocentrum lima, dinoflagellate species present in the Gulf of California, on marine bivalves, have demonstrated physiological alterations and changes in gene expression patterns; however, modification effects of the genes involved in immune defens...

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Autores principales:	García-Lagunas,Norma, Romero-Geraldo,Reyna, Kao-Godinez,Ana K., Hernández-Saavedra,Norma Y.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Crassostrea gigas Prorocentrum lima Gymnodinium catenatum dinoflagellates gene expression immune response
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000400699
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spelling	oai:scielo:S0718-560X20190004006992019-09-24Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum limaGarcía-Lagunas,NormaRomero-Geraldo,ReynaKao-Godinez,Ana K.Hernández-Saavedra,Norma Y. Crassostrea gigas Prorocentrum lima Gymnodinium catenatum dinoflagellates gene expression immune response ABSTRACT The effects of Gymnodynium catenatum and Prorocentrum lima, dinoflagellate species present in the Gulf of California, on marine bivalves, have demonstrated physiological alterations and changes in gene expression patterns; however, modification effects of the genes involved in immune defense are still poorly understood. This study analyzed the mRNA levels of five genes encoding for the immune defense of oyster Crassostrea gigas spat fed with a control diet (Isochrysis galbana alone) and a combination of the two toxic dinoflagellates. Expression levels of lipopolysaccharide (LPS)-binding protein 1,3-glucan and cavorting genes were higher in oysters exposed to the combined diets in all treatments compared to the non-toxic diet at day three, which was probably related with an activation of the oysters’ immediate immune response during the first 24 h. Protein 44 interferon-induced gene expression level was repressed in treatments with the highest dinoflagellate concentration and overexpressed in the diet with equal dinoflagellate concentration. Interaction protein-Toll and immunoglobulin gene transcript levels reached the highest values at day seven in oysters exposed to all combined diets. Then, the immune defense appeared to be activated in oyster spat as a response of toxins and/or extra-cellular compounds produced by the dinoflagellates.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.4 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000400699en10.3856/vol47-issue4-fulltext-12
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Crassostrea gigas Prorocentrum lima Gymnodinium catenatum dinoflagellates gene expression immune response
spellingShingle	Crassostrea gigas Prorocentrum lima Gymnodinium catenatum dinoflagellates gene expression immune response García-Lagunas,Norma Romero-Geraldo,Reyna Kao-Godinez,Ana K. Hernández-Saavedra,Norma Y. Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
description	ABSTRACT The effects of Gymnodynium catenatum and Prorocentrum lima, dinoflagellate species present in the Gulf of California, on marine bivalves, have demonstrated physiological alterations and changes in gene expression patterns; however, modification effects of the genes involved in immune defense are still poorly understood. This study analyzed the mRNA levels of five genes encoding for the immune defense of oyster Crassostrea gigas spat fed with a control diet (Isochrysis galbana alone) and a combination of the two toxic dinoflagellates. Expression levels of lipopolysaccharide (LPS)-binding protein 1,3-glucan and cavorting genes were higher in oysters exposed to the combined diets in all treatments compared to the non-toxic diet at day three, which was probably related with an activation of the oysters’ immediate immune response during the first 24 h. Protein 44 interferon-induced gene expression level was repressed in treatments with the highest dinoflagellate concentration and overexpressed in the diet with equal dinoflagellate concentration. Interaction protein-Toll and immunoglobulin gene transcript levels reached the highest values at day seven in oysters exposed to all combined diets. Then, the immune defense appeared to be activated in oyster spat as a response of toxins and/or extra-cellular compounds produced by the dinoflagellates.
author	García-Lagunas,Norma Romero-Geraldo,Reyna Kao-Godinez,Ana K. Hernández-Saavedra,Norma Y.
author_facet	García-Lagunas,Norma Romero-Geraldo,Reyna Kao-Godinez,Ana K. Hernández-Saavedra,Norma Y.
author_sort	García-Lagunas,Norma
title	Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
title_short	Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
title_full	Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
title_fullStr	Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
title_full_unstemmed	Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima
title_sort	differential expression of immune response genes in pacific oyster, crassostrea gigas spat, fed with dinoflagellates gymnodinium catenatum and prorocentrum lima
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000400699
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Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

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