Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review

ABSTRACT The trypsin enzyme of white shrimp Penaeus vannamei is a polymorphic molecule, which longest isolated and sequenced cDNA encoded a pre-proenzyme of 255 amino acids. Three of the described sequences are translated in the digestive gland, and SDS-PAGE detects the isoforms. The three isoforms...

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Autores principales:	Aguiñaga-Cruz,Jazmine Asusena, Sainz-Hernández,Juan Carlos, García-Rodríguez,Luis Daniel, García-Ulloa,Manuel, García-Gutiérrez,Cipriano, Montoya-Mejía,Magnolia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Penaeus vannamei trypsin modulation isotrypsins phenotype
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500723
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spelling	oai:scielo:S0718-560X20190005007232019-12-05Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a reviewAguiñaga-Cruz,Jazmine AsusenaSainz-Hernández,Juan CarlosGarcía-Rodríguez,Luis DanielGarcía-Ulloa,ManuelGarcía-Gutiérrez,CiprianoMontoya-Mejía,Magnolia Penaeus vannamei trypsin modulation isotrypsins phenotype ABSTRACT The trypsin enzyme of white shrimp Penaeus vannamei is a polymorphic molecule, which longest isolated and sequenced cDNA encoded a pre-proenzyme of 255 amino acids. Three of the described sequences are translated in the digestive gland, and SDS-PAGE detects the isoforms. The three isoforms named C, B and A are distributed by an individual to originate three trypsin phenotypes (CBA, CB and CA) that have already been isolated and characterized by defining theirs biochemical and kinetical differences. The CBA phenotypes exerted higher hydrolytic capabilities than the others. The trypsin phenotypes are inherited in a Mendelian fashion, and external or internal factors do not modulate them. In commercial hatcheries in Sinaloa, México, the most abundant phenotype was CBA, followed by CB and CA. The following two hypotheses may explain this finding: phenotype CA is not chosen throughout the selection process of breeders because they do not fit the anatomical characteristics desired by professionals in aquaculture or it is possible that shrimps with the CA phenotype die during the early stages of development. Two experimental designs were developed trying to explain such hypotheses and, in both experiments, CA phenotype frequency declined to zero when shrimps reached around 5 g of body weight. So far, no evidence explains the mortality of juvenile shrimps with phenotype CA. Research is needed to explain why CA phenotypes disappeared, how the survival of this phenotype can be improved, and how to produce shrimps with the most hydrolytic capability in support of the shrimp aquaculture industry.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.5 20192019-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500723en10.3856/vol47-issue5-fulltext-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Penaeus vannamei trypsin modulation isotrypsins phenotype
spellingShingle	Penaeus vannamei trypsin modulation isotrypsins phenotype Aguiñaga-Cruz,Jazmine Asusena Sainz-Hernández,Juan Carlos García-Rodríguez,Luis Daniel García-Ulloa,Manuel García-Gutiérrez,Cipriano Montoya-Mejía,Magnolia Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
description	ABSTRACT The trypsin enzyme of white shrimp Penaeus vannamei is a polymorphic molecule, which longest isolated and sequenced cDNA encoded a pre-proenzyme of 255 amino acids. Three of the described sequences are translated in the digestive gland, and SDS-PAGE detects the isoforms. The three isoforms named C, B and A are distributed by an individual to originate three trypsin phenotypes (CBA, CB and CA) that have already been isolated and characterized by defining theirs biochemical and kinetical differences. The CBA phenotypes exerted higher hydrolytic capabilities than the others. The trypsin phenotypes are inherited in a Mendelian fashion, and external or internal factors do not modulate them. In commercial hatcheries in Sinaloa, México, the most abundant phenotype was CBA, followed by CB and CA. The following two hypotheses may explain this finding: phenotype CA is not chosen throughout the selection process of breeders because they do not fit the anatomical characteristics desired by professionals in aquaculture or it is possible that shrimps with the CA phenotype die during the early stages of development. Two experimental designs were developed trying to explain such hypotheses and, in both experiments, CA phenotype frequency declined to zero when shrimps reached around 5 g of body weight. So far, no evidence explains the mortality of juvenile shrimps with phenotype CA. Research is needed to explain why CA phenotypes disappeared, how the survival of this phenotype can be improved, and how to produce shrimps with the most hydrolytic capability in support of the shrimp aquaculture industry.
author	Aguiñaga-Cruz,Jazmine Asusena Sainz-Hernández,Juan Carlos García-Rodríguez,Luis Daniel García-Ulloa,Manuel García-Gutiérrez,Cipriano Montoya-Mejía,Magnolia
author_facet	Aguiñaga-Cruz,Jazmine Asusena Sainz-Hernández,Juan Carlos García-Rodríguez,Luis Daniel García-Ulloa,Manuel García-Gutiérrez,Cipriano Montoya-Mejía,Magnolia
author_sort	Aguiñaga-Cruz,Jazmine Asusena
title	Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
title_short	Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
title_full	Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
title_fullStr	Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
title_full_unstemmed	Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review
title_sort	trypsin polymorphism and modulation in penaeus vannamei (boone, 1931): a review
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500723
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Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review

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