Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles

ABSTRACT This study assessed the effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles. Freshwater prawn juveniles (0.31 ± 0.004 g and 32.48 ± 0.27 mm) were fed diets with 20, 25, 30, 35 and 40% crude protein (CP) (p/p). In a sec...

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Autores principales:	Espinosa-Chaurand,Daniel, Carrillo-Farnés,Olimpia, Vega-Villasante,Fernando, Nolasco-Soria,Héctor
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Macrobrachium tenellum prawn feeding schedule digestive enzymes digestive physiology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500743
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spelling	oai:scielo:S0718-560X20190005007432019-12-05Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juvenilesEspinosa-Chaurand,DanielCarrillo-Farnés,OlimpiaVega-Villasante,FernandoNolasco-Soria,Héctor Macrobrachium tenellum prawn feeding schedule digestive enzymes digestive physiology ABSTRACT This study assessed the effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles. Freshwater prawn juveniles (0.31 ± 0.004 g and 32.48 ± 0.27 mm) were fed diets with 20, 25, 30, 35 and 40% crude protein (CP) (p/p). In a second experiment, feeding schedules were assessed at 08:00, 14:00 and 20:00 h with feed at 30% CP with juveniles of 0.22 ± 0.002 g and 29.36 ± 0.28 mm. In both experiments, five organisms were sampled after 60 days of treatment in a time-lapse of one hour (08:00 to 09:00 h) per experimental tank and treatment. The enzymatic extract was obtained from the complete organisms to determine specific general protease, trypsin, chymotrypsin, amylase and lipase activities. Greater general protease, trypsin and lipase activities were observed in feed at 40% CP. The feeding schedules with greater general protease, trypsin, lipase and amylase activities were found at 14:00 and 20:00 h. Chymotrypsin did not show differences among treatments at CP levels or feeding schedules. Protein level in diets for M. tenellum modified basal digestive enzymatic activity and increased proteolytic and lipolytic activities, while it decreased amylolytic activity as the inclusion of CP level increased in the diet; likewise, the activity was modified by feeding schedule, showing a direct correlation between the increase in digestive enzymatic activity and food offering at the end of the day.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.5 20192019-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500743en10.3856/vol47-issue5-fulltext-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Macrobrachium tenellum prawn feeding schedule digestive enzymes digestive physiology
spellingShingle	Macrobrachium tenellum prawn feeding schedule digestive enzymes digestive physiology Espinosa-Chaurand,Daniel Carrillo-Farnés,Olimpia Vega-Villasante,Fernando Nolasco-Soria,Héctor Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
description	ABSTRACT This study assessed the effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles. Freshwater prawn juveniles (0.31 ± 0.004 g and 32.48 ± 0.27 mm) were fed diets with 20, 25, 30, 35 and 40% crude protein (CP) (p/p). In a second experiment, feeding schedules were assessed at 08:00, 14:00 and 20:00 h with feed at 30% CP with juveniles of 0.22 ± 0.002 g and 29.36 ± 0.28 mm. In both experiments, five organisms were sampled after 60 days of treatment in a time-lapse of one hour (08:00 to 09:00 h) per experimental tank and treatment. The enzymatic extract was obtained from the complete organisms to determine specific general protease, trypsin, chymotrypsin, amylase and lipase activities. Greater general protease, trypsin and lipase activities were observed in feed at 40% CP. The feeding schedules with greater general protease, trypsin, lipase and amylase activities were found at 14:00 and 20:00 h. Chymotrypsin did not show differences among treatments at CP levels or feeding schedules. Protein level in diets for M. tenellum modified basal digestive enzymatic activity and increased proteolytic and lipolytic activities, while it decreased amylolytic activity as the inclusion of CP level increased in the diet; likewise, the activity was modified by feeding schedule, showing a direct correlation between the increase in digestive enzymatic activity and food offering at the end of the day.
author	Espinosa-Chaurand,Daniel Carrillo-Farnés,Olimpia Vega-Villasante,Fernando Nolasco-Soria,Héctor
author_facet	Espinosa-Chaurand,Daniel Carrillo-Farnés,Olimpia Vega-Villasante,Fernando Nolasco-Soria,Héctor
author_sort	Espinosa-Chaurand,Daniel
title	Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
title_short	Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
title_full	Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
title_fullStr	Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
title_full_unstemmed	Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles
title_sort	effect of protein level in diet and feeding schedule on the digestive enzymatic activity of macrobrachium tenellum juveniles
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500743
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Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles

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