Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta

ABSTRACT Navicula incerta is a benthic diatom with potential use in nutrition and health for humans and aquaculture. Therefore, it is important to know its optimal growth conditions and biochemical composition. In this study, the effects of nitrate and silicate concentration on the growth kinetics a...

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Autores principales: Encinas-Arzate,José Jesús, Marquez-Ríos,Enrique, López-Elías,José Antonio, Torres-Areola,Wilfrido, Huerta-Ocampo,José Ángel, Ramírez-Suárez,Juan Carlos
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2020
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200280
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spelling oai:scielo:S0718-560X20200002002802020-05-25Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incertaEncinas-Arzate,José JesúsMarquez-Ríos,EnriqueLópez-Elías,José AntonioTorres-Areola,WilfridoHuerta-Ocampo,José ÁngelRamírez-Suárez,Juan Carlos Navicula incerta stress response growth lipid production proximal composition nutrient limiting chlorophyll ABSTRACT Navicula incerta is a benthic diatom with potential use in nutrition and health for humans and aquaculture. Therefore, it is important to know its optimal growth conditions and biochemical composition. In this study, the effects of nitrate and silicate concentration on the growth kinetics and biochemical composition of N. incerta under laboratory conditions were measured. F/2 media was used as the control, and different concentrations of nitrogen (N/4, N/8 and N/16) and silicates (Si/4, Si/8 and Si/16) were evaluated. We measured cell concentration, chlorophyll-a and conducted proximal chemical analyses. It was found that different concentrations of nutrients affected the diatom's growth kinetics and affected the concentrations of ash, chlorophyll, protein, lipids and carbohydrates. The highest concentration of lipids was obtained in the limiting treatment of nitrogen N/8 (27.09%), while the lowest value was found with silicate Si/8 media (16.97%). Carbohydrates increased compared to the control, with the N/16 treatment presenting the maximal concentration (23.31%). Treatments with reduced nitrate (N/8 and N/16) demonstrated the lowest concentrations of protein (18.75 and 12.44%, respectively), while in reduced silicate treatments, no statistical differences (P ≥ 0.05) were observed. Therefore, media limited nitrogen and silicates affected the growth kinetics and proximal chemical composition of N. incerta. The growth of this species using the N/8 medium is a suitable method for increasing lipid concentration in N. incerta.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.48 n.2 20202020-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200280en10.3856/vol48-issue2-fulltext-2314
institution Scielo Chile
collection Scielo Chile
language English
topic Navicula incerta
stress response
growth
lipid production
proximal composition
nutrient limiting
chlorophyll
spellingShingle Navicula incerta
stress response
growth
lipid production
proximal composition
nutrient limiting
chlorophyll
Encinas-Arzate,José Jesús
Marquez-Ríos,Enrique
López-Elías,José Antonio
Torres-Areola,Wilfrido
Huerta-Ocampo,José Ángel
Ramírez-Suárez,Juan Carlos
Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
description ABSTRACT Navicula incerta is a benthic diatom with potential use in nutrition and health for humans and aquaculture. Therefore, it is important to know its optimal growth conditions and biochemical composition. In this study, the effects of nitrate and silicate concentration on the growth kinetics and biochemical composition of N. incerta under laboratory conditions were measured. F/2 media was used as the control, and different concentrations of nitrogen (N/4, N/8 and N/16) and silicates (Si/4, Si/8 and Si/16) were evaluated. We measured cell concentration, chlorophyll-a and conducted proximal chemical analyses. It was found that different concentrations of nutrients affected the diatom's growth kinetics and affected the concentrations of ash, chlorophyll, protein, lipids and carbohydrates. The highest concentration of lipids was obtained in the limiting treatment of nitrogen N/8 (27.09%), while the lowest value was found with silicate Si/8 media (16.97%). Carbohydrates increased compared to the control, with the N/16 treatment presenting the maximal concentration (23.31%). Treatments with reduced nitrate (N/8 and N/16) demonstrated the lowest concentrations of protein (18.75 and 12.44%, respectively), while in reduced silicate treatments, no statistical differences (P ≥ 0.05) were observed. Therefore, media limited nitrogen and silicates affected the growth kinetics and proximal chemical composition of N. incerta. The growth of this species using the N/8 medium is a suitable method for increasing lipid concentration in N. incerta.
author Encinas-Arzate,José Jesús
Marquez-Ríos,Enrique
López-Elías,José Antonio
Torres-Areola,Wilfrido
Huerta-Ocampo,José Ángel
Ramírez-Suárez,Juan Carlos
author_facet Encinas-Arzate,José Jesús
Marquez-Ríos,Enrique
López-Elías,José Antonio
Torres-Areola,Wilfrido
Huerta-Ocampo,José Ángel
Ramírez-Suárez,Juan Carlos
author_sort Encinas-Arzate,José Jesús
title Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
title_short Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
title_full Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
title_fullStr Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
title_full_unstemmed Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta
title_sort effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom navicula incerta
publisher Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200280
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