Screening of Chilean fish-killing microalgae using a gill cell-based assay

ABSTRACT Fish-killing algal species are responsible for important global economic losses to the finfish industry. Chile is the world’s second-largest salmon exporter, and fish-killing algal blooms have widely impacted its production in the last decades. The lack of standardized analytical...

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Autor principal:	Mardones,Jorge I.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2020
Materias:	harmful algal blooms salmon farming ichthyotoxic gill damage RTgill-W1 fish-kills Chilean fjords
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200329
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spelling	oai:scielo:S0718-560X20200002003292020-05-25Screening of Chilean fish-killing microalgae using a gill cell-based assayMardones,Jorge I. harmful algal blooms salmon farming ichthyotoxic gill damage RTgill-W1 fish-kills Chilean fjords ABSTRACT Fish-killing algal species are responsible for important global economic losses to the finfish industry. Chile is the world’s second-largest salmon exporter, and fish-killing algal blooms have widely impacted its production in the last decades. The lack of standardized analytical methods to quantify and characterize the so-called “ichthyotoxins” has hindered our understanding of the underlying ichthyotoxic modes of action. The novel application of a highly sensitive and reproducible fish RTgill-W1 cell line-based assay has allowed significant progress in the field. In this study, the ichthyotoxic potency of the main microalgae species, which has been reported in fish-killing events in the historical Chilean monitoring programs, was assessed. The dinoflagellate Karenia selliformis was the most ichthyotoxic species against the RTgill-W1 (cell viability down to 8%), representing the major threat for the local salmon industry. In comparison, the raphidophyte Heterosigma akashiwo and the dinoflagellate Prorocentrum micans were the least toxic (gill cell viability down to 81 and 89%, respectively). Importantly, ichthyotoxic flagellates were more toxic to fish gill cells upon rupture. These results have important implications for the mitigation and management of algal blooms by the salmon industry.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.48 n.2 20202020-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200329en10.3856/vol48-issue2-fulltext-2400
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	harmful algal blooms salmon farming ichthyotoxic gill damage RTgill-W1 fish-kills Chilean fjords
spellingShingle	harmful algal blooms salmon farming ichthyotoxic gill damage RTgill-W1 fish-kills Chilean fjords Mardones,Jorge I. Screening of Chilean fish-killing microalgae using a gill cell-based assay
description	ABSTRACT Fish-killing algal species are responsible for important global economic losses to the finfish industry. Chile is the world’s second-largest salmon exporter, and fish-killing algal blooms have widely impacted its production in the last decades. The lack of standardized analytical methods to quantify and characterize the so-called “ichthyotoxins” has hindered our understanding of the underlying ichthyotoxic modes of action. The novel application of a highly sensitive and reproducible fish RTgill-W1 cell line-based assay has allowed significant progress in the field. In this study, the ichthyotoxic potency of the main microalgae species, which has been reported in fish-killing events in the historical Chilean monitoring programs, was assessed. The dinoflagellate Karenia selliformis was the most ichthyotoxic species against the RTgill-W1 (cell viability down to 8%), representing the major threat for the local salmon industry. In comparison, the raphidophyte Heterosigma akashiwo and the dinoflagellate Prorocentrum micans were the least toxic (gill cell viability down to 81 and 89%, respectively). Importantly, ichthyotoxic flagellates were more toxic to fish gill cells upon rupture. These results have important implications for the mitigation and management of algal blooms by the salmon industry.
author	Mardones,Jorge I.
author_facet	Mardones,Jorge I.
author_sort	Mardones,Jorge I.
title	Screening of Chilean fish-killing microalgae using a gill cell-based assay
title_short	Screening of Chilean fish-killing microalgae using a gill cell-based assay
title_full	Screening of Chilean fish-killing microalgae using a gill cell-based assay
title_fullStr	Screening of Chilean fish-killing microalgae using a gill cell-based assay
title_full_unstemmed	Screening of Chilean fish-killing microalgae using a gill cell-based assay
title_sort	screening of chilean fish-killing microalgae using a gill cell-based assay
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000200329
work_keys_str_mv	AT mardonesjorgei screeningofchileanfishkillingmicroalgaeusingagillcellbasedassay
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Screening of Chilean fish-killing microalgae using a gill cell-based assay

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