High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

ABSTRACT Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impa...

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Autores principales:	Méndez-Pérez,Roberto, García-López,Rodrigo, Bautista-López,J. Santiago, Vázquez-Castellanos,Jorge, Alvarez-González,Carlos, Peña-Marín,Emyr, Baltierra-Trejo,Eduardo, Adams-Schroeder,Randy, Domínguez-Rodríguez,Verónica, Melgar-Valdés,Carolina, Martínez-García,Rafael, Moya,Andrés, Gómez-Cruz,Rodolfo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2020
Materias:	Atractosteus tropicus gut microbiome metagenomics 16S rRNA profiling
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000300456
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spelling	oai:scielo:S0718-560X20200003004562020-07-23High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)Méndez-Pérez,RobertoGarcía-López,RodrigoBautista-López,J. SantiagoVázquez-Castellanos,JorgeAlvarez-González,CarlosPeña-Marín,EmyrBaltierra-Trejo,EduardoAdams-Schroeder,RandyDomínguez-Rodríguez,VerónicaMelgar-Valdés,CarolinaMartínez-García,RafaelMoya,AndrésGómez-Cruz,Rodolfo Atractosteus tropicus gut microbiome metagenomics 16S rRNA profiling ABSTRACT Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951, Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.48 n.3 20202020-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000300456en10.3856/vol48-issue3-fulltext-2419
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Atractosteus tropicus gut microbiome metagenomics 16S rRNA profiling
spellingShingle	Atractosteus tropicus gut microbiome metagenomics 16S rRNA profiling Méndez-Pérez,Roberto García-López,Rodrigo Bautista-López,J. Santiago Vázquez-Castellanos,Jorge Alvarez-González,Carlos Peña-Marín,Emyr Baltierra-Trejo,Eduardo Adams-Schroeder,Randy Domínguez-Rodríguez,Verónica Melgar-Valdés,Carolina Martínez-García,Rafael Moya,Andrés Gómez-Cruz,Rodolfo High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
description	ABSTRACT Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951, Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.
author	Méndez-Pérez,Roberto García-López,Rodrigo Bautista-López,J. Santiago Vázquez-Castellanos,Jorge Alvarez-González,Carlos Peña-Marín,Emyr Baltierra-Trejo,Eduardo Adams-Schroeder,Randy Domínguez-Rodríguez,Verónica Melgar-Valdés,Carolina Martínez-García,Rafael Moya,Andrés Gómez-Cruz,Rodolfo
author_facet	Méndez-Pérez,Roberto García-López,Rodrigo Bautista-López,J. Santiago Vázquez-Castellanos,Jorge Alvarez-González,Carlos Peña-Marín,Emyr Baltierra-Trejo,Eduardo Adams-Schroeder,Randy Domínguez-Rodríguez,Verónica Melgar-Valdés,Carolina Martínez-García,Rafael Moya,Andrés Gómez-Cruz,Rodolfo
author_sort	Méndez-Pérez,Roberto
title	High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_short	High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_full	High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_fullStr	High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_full_unstemmed	High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_sort	high-throughput sequencing of the 16s rrna gene to analyze the gut microbiome in juvenile and adult tropical gar (atractosteus tropicus)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000300456
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High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

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