Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone

ABSTRACT The production of Nile tilapia (Oreochromis niloticus) has good technological development; however, today, it is still necessary to make it more efficient. One way to increase efficiency is to prevent disease and improve the food conversion factor. Since previous investigations of tilapia m...

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Autores principales:	Parra-Laca,Rocio, Hernández-Andrade,Laura, García-Espinosa,Gary, Loza-Rubio,Elizabeth
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2020
Materias:	Oreochromis niloticus Nile tilapia microbiota Actinomycetes seasonal variability aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000500758
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spelling	oai:scielo:S0718-560X20200005007582020-11-18Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zoneParra-Laca,RocioHernández-Andrade,LauraGarcía-Espinosa,GaryLoza-Rubio,Elizabeth Oreochromis niloticus Nile tilapia microbiota Actinomycetes seasonal variability aquaculture ABSTRACT The production of Nile tilapia (Oreochromis niloticus) has good technological development; however, today, it is still necessary to make it more efficient. One way to increase efficiency is to prevent disease and improve the food conversion factor. Since previous investigations of tilapia microbiota detected a high proportion of organisms belonging to the order Actinomycetes, this study was to isolate, identify, and describe the species of bacteria microbiota belonging to the cultured Nile tilapia. These were done with Nile tilapia grown in a warm sub-humid climate during spring and summer seasons. The biopsy of different organs was performed for bacteriological culture and 16S rRNA sequencing analysis. From the 180 tissue samples, 49 isolates of the order Actinomycetes were obtained, representing ten species from seven genera: Microbacterium, Brevibacterium, Cellulomonas, Corynebacterium, Kocuria, Actinomyces, and Micrococcus. In spring, Microbacterium dominated, accounting for 74% of the total population. In the summer, lower diversity was observed, with 39% represented by Microbacterium. 16S rRNA sequencing analysis enabled the classification of Actinomyces neuii and Microbacterium lacticum as Kocuria varians and Agromyces indicus; the classification of Microbacterium imperiale as Rhodococcus and Micrococcus luteus was confirmed. No sequences of K. varians have been reported in fish. Microbacterium dextranoliticum showed high similarity to environmental samples. Here is the first study that analyzes the bacteria population in tilapia at the genetic level with an ecosystem approach, present in healthy cultured tilapia, indicating their beneficial associations with the host, making them candidates as probiotics, among other possible functions, applicable in tilapia cultivation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.48 n.5 20202020-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000500758en10.3856/vol48-issue5-fulltext-2500
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Oreochromis niloticus Nile tilapia microbiota Actinomycetes seasonal variability aquaculture
spellingShingle	Oreochromis niloticus Nile tilapia microbiota Actinomycetes seasonal variability aquaculture Parra-Laca,Rocio Hernández-Andrade,Laura García-Espinosa,Gary Loza-Rubio,Elizabeth Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
description	ABSTRACT The production of Nile tilapia (Oreochromis niloticus) has good technological development; however, today, it is still necessary to make it more efficient. One way to increase efficiency is to prevent disease and improve the food conversion factor. Since previous investigations of tilapia microbiota detected a high proportion of organisms belonging to the order Actinomycetes, this study was to isolate, identify, and describe the species of bacteria microbiota belonging to the cultured Nile tilapia. These were done with Nile tilapia grown in a warm sub-humid climate during spring and summer seasons. The biopsy of different organs was performed for bacteriological culture and 16S rRNA sequencing analysis. From the 180 tissue samples, 49 isolates of the order Actinomycetes were obtained, representing ten species from seven genera: Microbacterium, Brevibacterium, Cellulomonas, Corynebacterium, Kocuria, Actinomyces, and Micrococcus. In spring, Microbacterium dominated, accounting for 74% of the total population. In the summer, lower diversity was observed, with 39% represented by Microbacterium. 16S rRNA sequencing analysis enabled the classification of Actinomyces neuii and Microbacterium lacticum as Kocuria varians and Agromyces indicus; the classification of Microbacterium imperiale as Rhodococcus and Micrococcus luteus was confirmed. No sequences of K. varians have been reported in fish. Microbacterium dextranoliticum showed high similarity to environmental samples. Here is the first study that analyzes the bacteria population in tilapia at the genetic level with an ecosystem approach, present in healthy cultured tilapia, indicating their beneficial associations with the host, making them candidates as probiotics, among other possible functions, applicable in tilapia cultivation.
author	Parra-Laca,Rocio Hernández-Andrade,Laura García-Espinosa,Gary Loza-Rubio,Elizabeth
author_facet	Parra-Laca,Rocio Hernández-Andrade,Laura García-Espinosa,Gary Loza-Rubio,Elizabeth
author_sort	Parra-Laca,Rocio
title	Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
title_short	Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
title_full	Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
title_fullStr	Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
title_full_unstemmed	Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone
title_sort	isolation of actinomycetes from nile tilapia (oreochromis niloticus) cultivated in semi-intensive systems from morelos, mexico central zone
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2020000500758
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Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone

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