Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination

ABSTRACT Vibrio parahaemolyticus is recognized as a human pathogen as well as the causative agent of vibriosis in shrimp. This study determined the pathogenic, antimicrobial, and biotic potential of V. parahaemolyticus isolated from white shrimp (Penaeus vannamei) and seawater on a northwestern Mexi...

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Autores principales:	Gámez-Bayardo,Sergio, Castañeda-Ruelas,Gloria Marisol, Espinosa-Plascencia,Angélica, Bermúdez-Almada,María del Carmen, Jiménez-Edeza,Maribel
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	Penaeus vannamei Vibrio parahaemolyticus phenotypes characterization survival aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100075
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spelling	oai:scielo:S0718-560X20210001000752021-03-22Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and disseminationGámez-Bayardo,SergioCastañeda-Ruelas,Gloria MarisolEspinosa-Plascencia,AngélicaBermúdez-Almada,María del CarmenJiménez-Edeza,Maribel Penaeus vannamei Vibrio parahaemolyticus phenotypes characterization survival aquaculture ABSTRACT Vibrio parahaemolyticus is recognized as a human pathogen as well as the causative agent of vibriosis in shrimp. This study determined the pathogenic, antimicrobial, and biotic potential of V. parahaemolyticus isolated from white shrimp (Penaeus vannamei) and seawater on a northwestern Mexico farm. A total of 140 samples were randomly collected, including juvenile organisms (n = 120) and seawater (n = 20). The pH, salinity, and biota of the ponds were used to correlate with bacterium presence. The strains were characterized by virulence genes presence, biofilm formation capacity, antimicrobial sensitivity, and the kinetics growth using PCR, microplates method, minimum inhibitory concentration (MIC), and spectrophotometry, respectively. V. parahaemolyticus was detected in 7.1% of the samples with a mean concentration of 3.72 1.24 log CFU mL-1; 6.7% (8/120) in shrimp and 10.0% (2/20) in seawater. Cultivation conditions were not predictive of the specie (P > 0.05). V. parahaemolyticus showed an adaptation time of 1.0 h, and a growth rate of 0.375 h−1 in seawater at 30°C. The strains were classified into two pathotypes: tlh+/tdh−/trh−/AP2− (75%) and tlh+/tdh−/trh−/AP2+ (25%) and three resistant profiles to clinical drugs (ampicillin, amikacin, gentamicin, and netilmicin). The MIC values against oxytetracycline (OTC), florfenicol (FFC) and enrofloxacin (ENRO) were >0.50, >0.25 and >0.06 μg mL−1, respectively. Biofilm formation was a property identified in 40% of the strains. The presence of infectious V. parahaemolyticus with high adaptative potential justifies integrating integrated aquaculture practices and management to control pathogen growth and shrimp health.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.1 20212021-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100075en10.3856/vol49-issue1-fulltext-2512
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Penaeus vannamei Vibrio parahaemolyticus phenotypes characterization survival aquaculture
spellingShingle	Penaeus vannamei Vibrio parahaemolyticus phenotypes characterization survival aquaculture Gámez-Bayardo,Sergio Castañeda-Ruelas,Gloria Marisol Espinosa-Plascencia,Angélica Bermúdez-Almada,María del Carmen Jiménez-Edeza,Maribel Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
description	ABSTRACT Vibrio parahaemolyticus is recognized as a human pathogen as well as the causative agent of vibriosis in shrimp. This study determined the pathogenic, antimicrobial, and biotic potential of V. parahaemolyticus isolated from white shrimp (Penaeus vannamei) and seawater on a northwestern Mexico farm. A total of 140 samples were randomly collected, including juvenile organisms (n = 120) and seawater (n = 20). The pH, salinity, and biota of the ponds were used to correlate with bacterium presence. The strains were characterized by virulence genes presence, biofilm formation capacity, antimicrobial sensitivity, and the kinetics growth using PCR, microplates method, minimum inhibitory concentration (MIC), and spectrophotometry, respectively. V. parahaemolyticus was detected in 7.1% of the samples with a mean concentration of 3.72 1.24 log CFU mL-1; 6.7% (8/120) in shrimp and 10.0% (2/20) in seawater. Cultivation conditions were not predictive of the specie (P > 0.05). V. parahaemolyticus showed an adaptation time of 1.0 h, and a growth rate of 0.375 h−1 in seawater at 30°C. The strains were classified into two pathotypes: tlh+/tdh−/trh−/AP2− (75%) and tlh+/tdh−/trh−/AP2+ (25%) and three resistant profiles to clinical drugs (ampicillin, amikacin, gentamicin, and netilmicin). The MIC values against oxytetracycline (OTC), florfenicol (FFC) and enrofloxacin (ENRO) were >0.50, >0.25 and >0.06 μg mL−1, respectively. Biofilm formation was a property identified in 40% of the strains. The presence of infectious V. parahaemolyticus with high adaptative potential justifies integrating integrated aquaculture practices and management to control pathogen growth and shrimp health.
author	Gámez-Bayardo,Sergio Castañeda-Ruelas,Gloria Marisol Espinosa-Plascencia,Angélica Bermúdez-Almada,María del Carmen Jiménez-Edeza,Maribel
author_facet	Gámez-Bayardo,Sergio Castañeda-Ruelas,Gloria Marisol Espinosa-Plascencia,Angélica Bermúdez-Almada,María del Carmen Jiménez-Edeza,Maribel
author_sort	Gámez-Bayardo,Sergio
title	Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
title_short	Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
title_full	Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
title_fullStr	Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
title_full_unstemmed	Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination
title_sort	characterization of vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of mexico, as risk predictors for its adaptation and dissemination
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100075
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Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination

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