Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis

ABSTRACT Biofilms strongly influence bivalve settlement patterns on artificial substrates; however, their structure and taxonomic composition remains a black box. We characterized a natural biofilm composition that exhibits a large settlement of larvae of the Peruvian scallop Argopecten purpuratus b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	González-Pazmiño,José, Pretell,Krizia Maribell, Zapata-Vidaurre,Karina, Mesones,Maribel Lucero, Quimí-Mujica,Juan, Diringer,Benoit
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	Argopecten purpuratus biofilm microbiota settlement larvae NGS LSCM
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100086
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-560X2021000100086
record_format	dspace
spelling	oai:scielo:S0718-560X20210001000862021-03-22Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysisGonzález-Pazmiño,JoséPretell,Krizia MaribellZapata-Vidaurre,KarinaMesones,Maribel LuceroQuimí-Mujica,JuanDiringer,Benoit Argopecten purpuratus biofilm microbiota settlement larvae NGS LSCM ABSTRACT Biofilms strongly influence bivalve settlement patterns on artificial substrates; however, their structure and taxonomic composition remains a black box. We characterized a natural biofilm composition that exhibits a large settlement of larvae of the Peruvian scallop Argopecten purpuratus by culture-dependent and culture-independent methods. Thirty-two different strains, representing six genera (10 strains of Bacillus, 9 of Vibrio, 6 Acinetobacter, 4 Staphylococcus, 2 Photobacterium, and 1 Exiguobacterium) were isolated. Those strains represented only 1.09% of the relative abundance compared with the total microbiota obtained by 16S rRNA high-throughput sequencing. The metagenomic analysis identified 441 species. Prokaryotes were predominant (93.4%) over eukaryotes (6.6%), with Pelobacter (13.4%), Lewinella (5.6%), Marinobacter (5.4%), Hoeflea (4.2%), and Microcystis (3.1%) being the most representative genera. Laser scanning confocal microscopy (LSCM) imaging evidenced an irregular and heterogeneous biofilm with an average thickness of 35 μm, where the heterotrophic prokaryotic community (3.4×106 cell cm−2) dominate the photoautotrophic communities (2.3×105 cell cm−2). For the first time, an A. purpuratus settlement-related biofilm was described by the next generation sequencing tool (NGS) and compared with traditional methodologies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.1 20212021-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100086en10.3856/vol49-issue1-fulltext-2547
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Argopecten purpuratus biofilm microbiota settlement larvae NGS LSCM
spellingShingle	Argopecten purpuratus biofilm microbiota settlement larvae NGS LSCM González-Pazmiño,José Pretell,Krizia Maribell Zapata-Vidaurre,Karina Mesones,Maribel Lucero Quimí-Mujica,Juan Diringer,Benoit Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
description	ABSTRACT Biofilms strongly influence bivalve settlement patterns on artificial substrates; however, their structure and taxonomic composition remains a black box. We characterized a natural biofilm composition that exhibits a large settlement of larvae of the Peruvian scallop Argopecten purpuratus by culture-dependent and culture-independent methods. Thirty-two different strains, representing six genera (10 strains of Bacillus, 9 of Vibrio, 6 Acinetobacter, 4 Staphylococcus, 2 Photobacterium, and 1 Exiguobacterium) were isolated. Those strains represented only 1.09% of the relative abundance compared with the total microbiota obtained by 16S rRNA high-throughput sequencing. The metagenomic analysis identified 441 species. Prokaryotes were predominant (93.4%) over eukaryotes (6.6%), with Pelobacter (13.4%), Lewinella (5.6%), Marinobacter (5.4%), Hoeflea (4.2%), and Microcystis (3.1%) being the most representative genera. Laser scanning confocal microscopy (LSCM) imaging evidenced an irregular and heterogeneous biofilm with an average thickness of 35 μm, where the heterotrophic prokaryotic community (3.4×106 cell cm−2) dominate the photoautotrophic communities (2.3×105 cell cm−2). For the first time, an A. purpuratus settlement-related biofilm was described by the next generation sequencing tool (NGS) and compared with traditional methodologies.
author	González-Pazmiño,José Pretell,Krizia Maribell Zapata-Vidaurre,Karina Mesones,Maribel Lucero Quimí-Mujica,Juan Diringer,Benoit
author_facet	González-Pazmiño,José Pretell,Krizia Maribell Zapata-Vidaurre,Karina Mesones,Maribel Lucero Quimí-Mujica,Juan Diringer,Benoit
author_sort	González-Pazmiño,José
title	Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
title_short	Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
title_full	Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
title_fullStr	Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
title_full_unstemmed	Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
title_sort	microbial characterization of a natural biofilm associated with peruvian scallop (argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000100086
work_keys_str_mv	AT gonzalezpazminojose microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis AT pretellkriziamaribell microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis AT zapatavidaurrekarina microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis AT mesonesmaribellucero microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis AT quimimujicajuan microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis AT diringerbenoit microbialcharacterizationofanaturalbiofilmassociatedwithperuvianscallopargopectenpurpuratuslarvaesettlementonartificialcollectorbyconfocalimagingmicrobiologyandmetagenomicanalysis
_version_	1714205245891936256

Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis

Ejemplares similares