Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei

ABSTRACT The effect of dietary inulin on the intestinal bacterial communities of Penaeus vannamei by 16S metagenomic analysis was assessed. PCR amplified the V3 region of the bacterial 16S rDNA. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Il...

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Autores principales:	Escamilla-Montes,Ruth, Barraza,Aarón, Luna-González,Antonio, Angulo,Carlos, Fierro-Coronado,Jesús Arturo, Diarte-Plata,Genaro, Flores-Miranda,Ma. del Carmen
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	Penaeus vannamei metagenome microbial network prebiotic shrimp gut aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000300418
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spelling	oai:scielo:S0718-560X20210003004182021-07-27Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannameiEscamilla-Montes,RuthBarraza,AarónLuna-González,AntonioAngulo,CarlosFierro-Coronado,Jesús ArturoDiarte-Plata,GenaroFlores-Miranda,Ma. del Carmen Penaeus vannamei metagenome microbial network prebiotic shrimp gut aquaculture ABSTRACT The effect of dietary inulin on the intestinal bacterial communities of Penaeus vannamei by 16S metagenomic analysis was assessed. PCR amplified the V3 region of the bacterial 16S rDNA. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The software Shaman and MicrobiomeAnalyst were used to analyze the sequences. The phylum Proteobacteria and the genus Vibrio were among the most abundant taxonomic ranks for control and inulin treatment. The relative abundance of the phylum Bacteroidetes and genus Ruegeria was lower in inulin treatment concerning the control condition. Alpha and beta indices did not show significant differences between inulin treatment and control conditions. For all samples, most of the bacterial organisms showed the presence of carbohydrate and amino acid metabolism-related genes, and to a lesser extent, of energy, lipid, and cofactors and vitamin metabolism-related genes. The principal metabolic functions were glycine, serine, threonine, glyoxylate and dicarboxylate, purine, pyrimidine, pyruvate, and quorum sensing. The interaction network analysis showed fewer interactions in the inulin treatment concerning control condition. Proteobacteria, Bacteroidetes, Vibrio, and Ruegeria predominated in all samples, and inulin did not change the net microbial diversity in the intestine of P. vannamei. Streptomyces, Roseobacter, and Ruegeria showed negative interactions with Vibrio, suggesting their use as probiotics. This study sheds light on the inulin supplement on the essential role of microbiota in the shrimp.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.3 20212021-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000300418en10.3856/vol49-issue3-fulltext-2653
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Penaeus vannamei metagenome microbial network prebiotic shrimp gut aquaculture
spellingShingle	Penaeus vannamei metagenome microbial network prebiotic shrimp gut aquaculture Escamilla-Montes,Ruth Barraza,Aarón Luna-González,Antonio Angulo,Carlos Fierro-Coronado,Jesús Arturo Diarte-Plata,Genaro Flores-Miranda,Ma. del Carmen Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
description	ABSTRACT The effect of dietary inulin on the intestinal bacterial communities of Penaeus vannamei by 16S metagenomic analysis was assessed. PCR amplified the V3 region of the bacterial 16S rDNA. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The software Shaman and MicrobiomeAnalyst were used to analyze the sequences. The phylum Proteobacteria and the genus Vibrio were among the most abundant taxonomic ranks for control and inulin treatment. The relative abundance of the phylum Bacteroidetes and genus Ruegeria was lower in inulin treatment concerning the control condition. Alpha and beta indices did not show significant differences between inulin treatment and control conditions. For all samples, most of the bacterial organisms showed the presence of carbohydrate and amino acid metabolism-related genes, and to a lesser extent, of energy, lipid, and cofactors and vitamin metabolism-related genes. The principal metabolic functions were glycine, serine, threonine, glyoxylate and dicarboxylate, purine, pyrimidine, pyruvate, and quorum sensing. The interaction network analysis showed fewer interactions in the inulin treatment concerning control condition. Proteobacteria, Bacteroidetes, Vibrio, and Ruegeria predominated in all samples, and inulin did not change the net microbial diversity in the intestine of P. vannamei. Streptomyces, Roseobacter, and Ruegeria showed negative interactions with Vibrio, suggesting their use as probiotics. This study sheds light on the inulin supplement on the essential role of microbiota in the shrimp.
author	Escamilla-Montes,Ruth Barraza,Aarón Luna-González,Antonio Angulo,Carlos Fierro-Coronado,Jesús Arturo Diarte-Plata,Genaro Flores-Miranda,Ma. del Carmen
author_facet	Escamilla-Montes,Ruth Barraza,Aarón Luna-González,Antonio Angulo,Carlos Fierro-Coronado,Jesús Arturo Diarte-Plata,Genaro Flores-Miranda,Ma. del Carmen
author_sort	Escamilla-Montes,Ruth
title	Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
title_short	Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
title_full	Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
title_fullStr	Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
title_full_unstemmed	Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei
title_sort	effect of dietary inulin in the gut microbiota of whiteleg shrimp penaeus vannamei
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000300418
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Effect of dietary inulin in the gut microbiota of whiteleg shrimp Penaeus vannamei

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