Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae

ABSTRACT One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numero...

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Autores principales:	Savin-Amador,Macario, Rojas-Contreras,Maurilia, Arce-Amézquita,Pablo Misael, Rangel-Dávalos,Carlos, Vázquez-Juárez,Ricardo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	Crassostrea gigas larvae Lactobacillus plantarum settlement probiotic aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400551
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spelling	oai:scielo:S0718-560X20210004005512021-09-27Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvaeSavin-Amador,MacarioRojas-Contreras,MauriliaArce-Amézquita,Pablo MisaelRangel-Dávalos,CarlosVázquez-Juárez,Ricardo Crassostrea gigas larvae Lactobacillus plantarum settlement probiotic aquaculture ABSTRACT One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numerous studies indicate that before larval settlement to a substrate, they require bacteria to stimulate and induce the process. In this study, Japanese oyster (Crassostrea gigas) larvae were fed with combinations of microalgae and bacteria previously selected for their probiotic potential; their impact on survival, growth, and settlement was evaluated. Larvae D of 12 days old were provided by a private company and transported to the laboratory under controlled conditions. Probiotic bacteria Lactobacillus plantarum 69Cr, L. fermentum 101Cc, and L. casei 43Cg were reactivated on MRS and applied at a concentration of 1×104 CFU mL−1. Microalgae Isochrysis galbana and Chaetoceros calcitrans were grown in F2 medium and supplied at a concentration of 3×104 cells mL−1. Results showed, in all treatments, a survival range of 39-53%, growth of 300-310 μm, and 34-56.5% settlement. However, the best bacteria combined with I. galbana and C. calcitrans was L. plantarum 69Cr with a 54% survival, 310 μm growth, and 56.5% settlement. It is concluded that the combination of L. plantarum 69Cr with I. galbana and C. calcitrans has the potential to be used in the production of Japanese oyster larvae.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.4 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400551en10.3856/vol49-issue4-fulltext-2672
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Crassostrea gigas larvae Lactobacillus plantarum settlement probiotic aquaculture
spellingShingle	Crassostrea gigas larvae Lactobacillus plantarum settlement probiotic aquaculture Savin-Amador,Macario Rojas-Contreras,Maurilia Arce-Amézquita,Pablo Misael Rangel-Dávalos,Carlos Vázquez-Juárez,Ricardo Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
description	ABSTRACT One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numerous studies indicate that before larval settlement to a substrate, they require bacteria to stimulate and induce the process. In this study, Japanese oyster (Crassostrea gigas) larvae were fed with combinations of microalgae and bacteria previously selected for their probiotic potential; their impact on survival, growth, and settlement was evaluated. Larvae D of 12 days old were provided by a private company and transported to the laboratory under controlled conditions. Probiotic bacteria Lactobacillus plantarum 69Cr, L. fermentum 101Cc, and L. casei 43Cg were reactivated on MRS and applied at a concentration of 1×104 CFU mL−1. Microalgae Isochrysis galbana and Chaetoceros calcitrans were grown in F2 medium and supplied at a concentration of 3×104 cells mL−1. Results showed, in all treatments, a survival range of 39-53%, growth of 300-310 μm, and 34-56.5% settlement. However, the best bacteria combined with I. galbana and C. calcitrans was L. plantarum 69Cr with a 54% survival, 310 μm growth, and 56.5% settlement. It is concluded that the combination of L. plantarum 69Cr with I. galbana and C. calcitrans has the potential to be used in the production of Japanese oyster larvae.
author	Savin-Amador,Macario Rojas-Contreras,Maurilia Arce-Amézquita,Pablo Misael Rangel-Dávalos,Carlos Vázquez-Juárez,Ricardo
author_facet	Savin-Amador,Macario Rojas-Contreras,Maurilia Arce-Amézquita,Pablo Misael Rangel-Dávalos,Carlos Vázquez-Juárez,Ricardo
author_sort	Savin-Amador,Macario
title	Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
title_short	Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
title_full	Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
title_fullStr	Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
title_full_unstemmed	Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae
title_sort	lactobacillus strains isolated from oysters improve the production of crassostrea gigas larvae
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400551
work_keys_str_mv	AT savinamadormacario lactobacillusstrainsisolatedfromoystersimprovetheproductionofcrassostreagigaslarvae AT rojascontrerasmaurilia lactobacillusstrainsisolatedfromoystersimprovetheproductionofcrassostreagigaslarvae AT arceamezquitapablomisael lactobacillusstrainsisolatedfromoystersimprovetheproductionofcrassostreagigaslarvae AT rangeldavaloscarlos lactobacillusstrainsisolatedfromoystersimprovetheproductionofcrassostreagigaslarvae AT vazquezjuarezricardo lactobacillusstrainsisolatedfromoystersimprovetheproductionofcrassostreagigaslarvae
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Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae

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