Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)

ABSTRACT The histological structure, histochemical features, and enzymatic activity of the digestive tract of juvenile Pacific seahorse (Hippocampus ingens) are described to provide information during the cultivation of this species. Serial histological sections were stained with either hematoxylin-...

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Autores principales:	Corona-Rojas,Daniela, Peña,Renato, Rodríguez-Jaramillo,Carmen, Tovar-Ramírez,Dariel, Hinojosa-Baltazar,Patricia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	Hippocampus ingens Pacific seahorse digestive tract histological structure enzymatic activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400565
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spelling	oai:scielo:S0718-560X20210004005652021-09-27Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)Corona-Rojas,DanielaPeña,RenatoRodríguez-Jaramillo,CarmenTovar-Ramírez,DarielHinojosa-Baltazar,Patricia Hippocampus ingens Pacific seahorse digestive tract histological structure enzymatic activity ABSTRACT The histological structure, histochemical features, and enzymatic activity of the digestive tract of juvenile Pacific seahorse (Hippocampus ingens) are described to provide information during the cultivation of this species. Serial histological sections were stained with either hematoxylin-eosin, alcian blue-PAS, toluidine blue, Sudan black, Masson's trichome, and ninhydrin-Schiff to describe the general features and the presence of glycogen, mucopolysaccharides, lipids, muscle layers, and proteins, respectively. The enterocytes height and the mucosal villi height in the esophagus and intestines were measured. Additionally, the digestive enzymes trypsin, chymotrypsin, lipase, amylase, aminopeptidase, acid phosphatase, and alkaline phosphatase activities were recorded. The esophagus showed two distinctive regions, the anterior with numerous mucous cells secreting acid mucins and the posterior with longitudinal folds and no mucous cells. The intestine was differentiated into three regions. The anterior showed goblet cells secreting acid and neutral mucins, while the middle and posterior regions presented goblet cells secreting only acid mucins. The activity of aminopeptidase, chymotrypsin, and amylase showed low levels, while the trypsin and acid phosphatase activity levels were intermediate. Lipase and alkaline phosphatase showed the highest activities. The results point that juvenile H. ingens presents a digestive structure similar to other teleost species. The high levels of lipase suggest that juvenile H. ingens have high requirements for lipids during this stage.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.4 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400565en10.3856/vol49-issue4-fulltext-2673
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hippocampus ingens Pacific seahorse digestive tract histological structure enzymatic activity
spellingShingle	Hippocampus ingens Pacific seahorse digestive tract histological structure enzymatic activity Corona-Rojas,Daniela Peña,Renato Rodríguez-Jaramillo,Carmen Tovar-Ramírez,Dariel Hinojosa-Baltazar,Patricia Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
description	ABSTRACT The histological structure, histochemical features, and enzymatic activity of the digestive tract of juvenile Pacific seahorse (Hippocampus ingens) are described to provide information during the cultivation of this species. Serial histological sections were stained with either hematoxylin-eosin, alcian blue-PAS, toluidine blue, Sudan black, Masson's trichome, and ninhydrin-Schiff to describe the general features and the presence of glycogen, mucopolysaccharides, lipids, muscle layers, and proteins, respectively. The enterocytes height and the mucosal villi height in the esophagus and intestines were measured. Additionally, the digestive enzymes trypsin, chymotrypsin, lipase, amylase, aminopeptidase, acid phosphatase, and alkaline phosphatase activities were recorded. The esophagus showed two distinctive regions, the anterior with numerous mucous cells secreting acid mucins and the posterior with longitudinal folds and no mucous cells. The intestine was differentiated into three regions. The anterior showed goblet cells secreting acid and neutral mucins, while the middle and posterior regions presented goblet cells secreting only acid mucins. The activity of aminopeptidase, chymotrypsin, and amylase showed low levels, while the trypsin and acid phosphatase activity levels were intermediate. Lipase and alkaline phosphatase showed the highest activities. The results point that juvenile H. ingens presents a digestive structure similar to other teleost species. The high levels of lipase suggest that juvenile H. ingens have high requirements for lipids during this stage.
author	Corona-Rojas,Daniela Peña,Renato Rodríguez-Jaramillo,Carmen Tovar-Ramírez,Dariel Hinojosa-Baltazar,Patricia
author_facet	Corona-Rojas,Daniela Peña,Renato Rodríguez-Jaramillo,Carmen Tovar-Ramírez,Dariel Hinojosa-Baltazar,Patricia
author_sort	Corona-Rojas,Daniela
title	Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
title_short	Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
title_full	Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
title_fullStr	Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
title_full_unstemmed	Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)
title_sort	histological structure of the digestive tract and digestive enzymatic activity of juvenile pacific seahorse (hippocampus ingens)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000400565
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Histological structure of the digestive tract and digestive enzymatic activity of juvenile Pacific seahorse (Hippocampus ingens)

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