Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus

ABSTRACT This work looks at the antibacterial efficacy of Gracilaria vermiculophylla and Ulva flexuosa aqueous extracts when added to the feed of Penaeus vannamei in an experimental infection with Vibrio parahaemolyticus (M0904AHPND+strain). Results show that the minimum inhibitory concentration (MI...

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Autores principales:	Gamboa-Barraza,Lizbeth Guadalupe, Bolan-Mejía,María del Carmen, Osuna-Ruiz,Idalia, Martínez-Rodríguez,Irma Eugenia, Calvario-Martínez,Omar, Morales-Covarrubias,María Soledad
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2021
Materias:	macroalgae Vibrio parahaemolyticus Penaeus vannamei phytogenics AHPND flavonoids
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000500739
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spelling	oai:scielo:S0718-560X20210005007392021-11-25Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticusGamboa-Barraza,Lizbeth GuadalupeBolan-Mejía,María del CarmenOsuna-Ruiz,IdaliaMartínez-Rodríguez,Irma EugeniaCalvario-Martínez,OmarMorales-Covarrubias,María Soledad macroalgae Vibrio parahaemolyticus Penaeus vannamei phytogenics AHPND flavonoids ABSTRACT This work looks at the antibacterial efficacy of Gracilaria vermiculophylla and Ulva flexuosa aqueous extracts when added to the feed of Penaeus vannamei in an experimental infection with Vibrio parahaemolyticus (M0904AHPND+strain). Results show that the minimum inhibitory concentration (MIC) for both extracts was 50 mg mL-1, with inhibition zones of 18.00 ± 0.60 mm for G. vermiculophylla and 14.00 ± 0.29 mm for U. flexuosa. G. vermiculophylla gave a higher total content of phenolic compounds (10.58 ± 2.31 mg GAE g-1) (gallic acid equivalent) and lower flavonoid content (10.32 ± 0.73 mg QE g-1) in comparison to U. flexuosa, while using ABTS (2,2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) to measure antioxidant capacity showed that G. vermiculophylla gave a better result than U. flexuosa. The challenge with V. parahaemolyticus resulted in 67% survival for organisms fed G. vermiculophylla and 60% for those fed U. flexuosa, resulting in twice the amount of survival as opposed to 30% in the positive control at 24 h post-infection. Histopathological alterations in the hepatopancreas with hemocytic infiltration within the intertubular connective tissue were observed. Also, tubules with severe cell detachment and tubular atrophy were detected in the positive control organisms, and organisms treated with macroalgae only had vermiform structures in the tubular lumen, cell detachment, and infiltration hemolymph in intertubular connective tissue. According to the analysis of the studied variables, it can be concluded that the aqueous extracts of these macroalgae are a promising alternative for V. parahaemolyticus (M0904AHPND+strain) control in shrimp culture.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.49 n.5 20212021-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000500739en10.3856/vol49-issue5-fulltext-2724
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	macroalgae Vibrio parahaemolyticus Penaeus vannamei phytogenics AHPND flavonoids
spellingShingle	macroalgae Vibrio parahaemolyticus Penaeus vannamei phytogenics AHPND flavonoids Gamboa-Barraza,Lizbeth Guadalupe Bolan-Mejía,María del Carmen Osuna-Ruiz,Idalia Martínez-Rodríguez,Irma Eugenia Calvario-Martínez,Omar Morales-Covarrubias,María Soledad Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
description	ABSTRACT This work looks at the antibacterial efficacy of Gracilaria vermiculophylla and Ulva flexuosa aqueous extracts when added to the feed of Penaeus vannamei in an experimental infection with Vibrio parahaemolyticus (M0904AHPND+strain). Results show that the minimum inhibitory concentration (MIC) for both extracts was 50 mg mL-1, with inhibition zones of 18.00 ± 0.60 mm for G. vermiculophylla and 14.00 ± 0.29 mm for U. flexuosa. G. vermiculophylla gave a higher total content of phenolic compounds (10.58 ± 2.31 mg GAE g-1) (gallic acid equivalent) and lower flavonoid content (10.32 ± 0.73 mg QE g-1) in comparison to U. flexuosa, while using ABTS (2,2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) to measure antioxidant capacity showed that G. vermiculophylla gave a better result than U. flexuosa. The challenge with V. parahaemolyticus resulted in 67% survival for organisms fed G. vermiculophylla and 60% for those fed U. flexuosa, resulting in twice the amount of survival as opposed to 30% in the positive control at 24 h post-infection. Histopathological alterations in the hepatopancreas with hemocytic infiltration within the intertubular connective tissue were observed. Also, tubules with severe cell detachment and tubular atrophy were detected in the positive control organisms, and organisms treated with macroalgae only had vermiform structures in the tubular lumen, cell detachment, and infiltration hemolymph in intertubular connective tissue. According to the analysis of the studied variables, it can be concluded that the aqueous extracts of these macroalgae are a promising alternative for V. parahaemolyticus (M0904AHPND+strain) control in shrimp culture.
author	Gamboa-Barraza,Lizbeth Guadalupe Bolan-Mejía,María del Carmen Osuna-Ruiz,Idalia Martínez-Rodríguez,Irma Eugenia Calvario-Martínez,Omar Morales-Covarrubias,María Soledad
author_facet	Gamboa-Barraza,Lizbeth Guadalupe Bolan-Mejía,María del Carmen Osuna-Ruiz,Idalia Martínez-Rodríguez,Irma Eugenia Calvario-Martínez,Omar Morales-Covarrubias,María Soledad
author_sort	Gamboa-Barraza,Lizbeth Guadalupe
title	Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
title_short	Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
title_full	Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
title_fullStr	Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
title_full_unstemmed	Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus
title_sort	evaluation of aqueous extracts of gracilaria vermiculophylla and ulva flexuosa as treatment in challenged penaeus vannamei with vibrio parahaemolyticus
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2021000500739
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Evaluation of aqueous extracts of Gracilaria vermiculophylla and Ulva flexuosa as treatment in challenged Penaeus vannamei with Vibrio parahaemolyticus

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