Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)

Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and intr...

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Autores principales:	Paredes C,Mario, Becerra V,Viviana, González A,María I
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2008
Materias:	germplasm genetic diversity RAPD diversity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100001
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spelling	oai:scielo:S0718-583920080001000012018-10-01Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)Paredes C,MarioBecerra V,VivianaGonzález A,María I germplasm genetic diversity RAPD diversity Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristicsinfo:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.68 n.1 20082008-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100001en10.4067/S0718-58392008000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	germplasm genetic diversity RAPD diversity
spellingShingle	germplasm genetic diversity RAPD diversity Paredes C,Mario Becerra V,Viviana González A,María I Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
description	Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristics
author	Paredes C,Mario Becerra V,Viviana González A,María I
author_facet	Paredes C,Mario Becerra V,Viviana González A,María I
author_sort	Paredes C,Mario
title	Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
title_short	Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
title_full	Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
title_fullStr	Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
title_full_unstemmed	Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
title_sort	low genetic diversity among garlic (allium sativum l.) accessions detected using random amplified polymorphic dna (rapd)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100001
work_keys_str_mv	AT paredescmario lowgeneticdiversityamonggarlicalliumsativumlaccessionsdetectedusingrandomamplifiedpolymorphicdnarapd AT becerravviviana lowgeneticdiversityamonggarlicalliumsativumlaccessionsdetectedusingrandomamplifiedpolymorphicdnarapd AT gonzalezamariai lowgeneticdiversityamonggarlicalliumsativumlaccessionsdetectedusingrandomamplifiedpolymorphicdnarapd
_version_	1714205257759719424

Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)

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