Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)

The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, ri...

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Autores principales:	Barrueto Cid,L. Pedro, Carvalho,Luiz L.C.B
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2008
Materias:	tissue culture dormancy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300011
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spelling	oai:scielo:S0718-583920080003000112018-10-01Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)Barrueto Cid,L. PedroCarvalho,Luiz L.C.B tissue culture dormancy The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers. We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA) concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium. Nodal explants were stored for three months with ABA. Plants were obtained after nodal axillary buds were placed in SP medium without ABA. Results indicated that 20 and 30 mM ABA induced bud dormancy and delayed sprouting without affecting subsequent growth of plants after treatment.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.68 n.3 20082008-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300011en10.4067/S0718-58392008000300011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	tissue culture dormancy
spellingShingle	tissue culture dormancy Barrueto Cid,L. Pedro Carvalho,Luiz L.C.B Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
description	The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers. We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA) concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium. Nodal explants were stored for three months with ABA. Plants were obtained after nodal axillary buds were placed in SP medium without ABA. Results indicated that 20 and 30 mM ABA induced bud dormancy and delayed sprouting without affecting subsequent growth of plants after treatment.
author	Barrueto Cid,L. Pedro Carvalho,Luiz L.C.B
author_facet	Barrueto Cid,L. Pedro Carvalho,Luiz L.C.B
author_sort	Barrueto Cid,L. Pedro
title	Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
title_short	Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
title_full	Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
title_fullStr	Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
title_full_unstemmed	Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
title_sort	importance of abscisic acid (aba) in the in vitro conservation of cassava (manihot esculenta crantz)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300011
work_keys_str_mv	AT barruetocidlpedro importanceofabscisicacidabaintheinvitroconservationofcassavamanihotesculentacrantz AT carvalholuizlcb importanceofabscisicacidabaintheinvitroconservationofcassavamanihotesculentacrantz
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Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)

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