Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid

From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8...

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Autores principales:	Sánchez-Olate,Manuel, Sáez,Patricia, Cartes,Priscila, Alvarez,Carolina, Ríos,Darcy
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	rooting European hazelnut
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013
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spelling	oai:scielo:S0718-583920090001000132018-10-01Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric AcidSánchez-Olate,ManuelSáez,PatriciaCartes,PriscilaAlvarez,CarolinaRíos,Darcy rooting European hazelnut From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results indicate that in the 16:8 h photoperiod induction, the rooting rate with IBA (90%) was significantly higher than that obtained with the strain A477 of A. rhizogenes (67.7%), while with the strain A478 no statistically significant difference was obtained for the same variable (75%). On the other hand, under complete darkness, rooting mediated by IBA (90%) significantly surpassed the results obtained with both strains of A. rhizogenes (40 and 20%, for A478 and A477, respectively). In terms of the morphological variables of the resulting root system, induction mediated by IBA, with a 16:8 h photoperiod, generates a significantly higher number of roots (19 roots per microshoot) than that obtained with A. rhizogenes (mean 3.7 roots per microshoot), producing significant differences when comparing the results with the strain A478 (5 roots per microshoot) to those of the strain A477 (2.4 roots per microshoot). Induction under complete darkness does not have any effect on root number, independent of the rhizogenic inductor employed. Root length did not present significant differences among treatments, except in the presence of A. rhizogenes A477 and darkness.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.1 20092009-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013en10.4067/S0718-58392009000100013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	rooting European hazelnut
spellingShingle	rooting European hazelnut Sánchez-Olate,Manuel Sáez,Patricia Cartes,Priscila Alvarez,Carolina Ríos,Darcy Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
description	From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results indicate that in the 16:8 h photoperiod induction, the rooting rate with IBA (90%) was significantly higher than that obtained with the strain A477 of A. rhizogenes (67.7%), while with the strain A478 no statistically significant difference was obtained for the same variable (75%). On the other hand, under complete darkness, rooting mediated by IBA (90%) significantly surpassed the results obtained with both strains of A. rhizogenes (40 and 20%, for A478 and A477, respectively). In terms of the morphological variables of the resulting root system, induction mediated by IBA, with a 16:8 h photoperiod, generates a significantly higher number of roots (19 roots per microshoot) than that obtained with A. rhizogenes (mean 3.7 roots per microshoot), producing significant differences when comparing the results with the strain A478 (5 roots per microshoot) to those of the strain A477 (2.4 roots per microshoot). Induction under complete darkness does not have any effect on root number, independent of the rhizogenic inductor employed. Root length did not present significant differences among treatments, except in the presence of A. rhizogenes A477 and darkness.
author	Sánchez-Olate,Manuel Sáez,Patricia Cartes,Priscila Alvarez,Carolina Ríos,Darcy
author_facet	Sánchez-Olate,Manuel Sáez,Patricia Cartes,Priscila Alvarez,Carolina Ríos,Darcy
author_sort	Sánchez-Olate,Manuel
title	Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
title_short	Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
title_full	Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
title_fullStr	Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
title_full_unstemmed	Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid
title_sort	comparison of root induction in mature filbert (corylus avellana l.) explants by agrobacterium rhizogenes and indolbutiric acid
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013
work_keys_str_mv	AT sanchezolatemanuel comparisonofrootinductioninmaturefilbertcorylusavellanalexplantsbyagrobacteriumrhizogenesandindolbutiricacid AT saezpatricia comparisonofrootinductioninmaturefilbertcorylusavellanalexplantsbyagrobacteriumrhizogenesandindolbutiricacid AT cartespriscila comparisonofrootinductioninmaturefilbertcorylusavellanalexplantsbyagrobacteriumrhizogenesandindolbutiricacid AT alvarezcarolina comparisonofrootinductioninmaturefilbertcorylusavellanalexplantsbyagrobacteriumrhizogenesandindolbutiricacid AT riosdarcy comparisonofrootinductioninmaturefilbertcorylusavellanalexplantsbyagrobacteriumrhizogenesandindolbutiricacid
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Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid

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