Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species

Regions traditionally destined to agriculture report an ever increasing exposure to cold and drought conditions. This is especially important in countries like Chile where crop management options are limited. The development of new cultivars with better yields under adverse conditions is fundamental...

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Autores principales:	Pérez-Torres,Eduardo, Paredes C,Mario, Polanco,Víctor, Becerra B,Viviana
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	cold drought crops species gene expression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200016
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spelling	oai:scielo:S0718-583920090002000162018-10-01Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops SpeciesPérez-Torres,EduardoParedes C,MarioPolanco,VíctorBecerra B,Viviana cold drought crops species gene expression Regions traditionally destined to agriculture report an ever increasing exposure to cold and drought conditions. This is especially important in countries like Chile where crop management options are limited. The development of new cultivars with better yields under adverse conditions is fundamental if the ever increasing demand for food is to be matched; however, improving tolerance to abiotic stresses has proved to be a complex task. In this regard, development in plant physiology and genomics in the last 20 years has led to a deeper understanding of how plants respond to stress and mechanisms responsible for different ranges of tolerance observed in nature. This review discusses the techniques currently most in use in gene expression analysis, together with some important experimental design variables, such as the developmental stage of the plant, stress intensity and duration, and how different stresses may interact when performing assays. On the other hand, it is fundamental to properly select gene expression techniques according to the available information on the genome, the crop and the final objective of the research. All these points must be considered to ease transition from genomics to practical applications to crop species in order to increase their tolerance to stress. In this regard, the rapid development of new techniques in gene expression analysis with lower costs will determine a new revolution in crop research in coming decades. Therefore, Chile needs to be prepared in this area to continue its development as a major food producer worldwide.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.2 20092009-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200016en10.4067/S0718-58392009000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cold drought crops species gene expression
spellingShingle	cold drought crops species gene expression Pérez-Torres,Eduardo Paredes C,Mario Polanco,Víctor Becerra B,Viviana Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
description	Regions traditionally destined to agriculture report an ever increasing exposure to cold and drought conditions. This is especially important in countries like Chile where crop management options are limited. The development of new cultivars with better yields under adverse conditions is fundamental if the ever increasing demand for food is to be matched; however, improving tolerance to abiotic stresses has proved to be a complex task. In this regard, development in plant physiology and genomics in the last 20 years has led to a deeper understanding of how plants respond to stress and mechanisms responsible for different ranges of tolerance observed in nature. This review discusses the techniques currently most in use in gene expression analysis, together with some important experimental design variables, such as the developmental stage of the plant, stress intensity and duration, and how different stresses may interact when performing assays. On the other hand, it is fundamental to properly select gene expression techniques according to the available information on the genome, the crop and the final objective of the research. All these points must be considered to ease transition from genomics to practical applications to crop species in order to increase their tolerance to stress. In this regard, the rapid development of new techniques in gene expression analysis with lower costs will determine a new revolution in crop research in coming decades. Therefore, Chile needs to be prepared in this area to continue its development as a major food producer worldwide.
author	Pérez-Torres,Eduardo Paredes C,Mario Polanco,Víctor Becerra B,Viviana
author_facet	Pérez-Torres,Eduardo Paredes C,Mario Polanco,Víctor Becerra B,Viviana
author_sort	Pérez-Torres,Eduardo
title	Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
title_short	Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
title_full	Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
title_fullStr	Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
title_full_unstemmed	Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species
title_sort	gene expression analysis: a way to study tolerance to abiotic stresses in crops species
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200016
work_keys_str_mv	AT pereztorreseduardo geneexpressionanalysisawaytostudytolerancetoabioticstressesincropsspecies AT paredescmario geneexpressionanalysisawaytostudytolerancetoabioticstressesincropsspecies AT polancovictor geneexpressionanalysisawaytostudytolerancetoabioticstressesincropsspecies AT becerrabviviana geneexpressionanalysisawaytostudytolerancetoabioticstressesincropsspecies
_version_	1714205269813100544

Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species

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