Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes

The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was...

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Autores principales:	Sánchez-Olate,Manuel, Sáez,Patricia, Ríos,Darcy
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	rooting microshoots adult material
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019
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spelling	oai:scielo:S0718-583920090002000192018-10-01Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenesSánchez-Olate,ManuelSáez,PatriciaRíos,Darcy rooting microshoots adult material The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase of root induction was during 3 days in the dark while the second phase, root manifestation, was 27 days. Rooting percentage was evaluated and the induced root systems characterized (number, length, diameter, and root insertion zone) in all the procedures. The best rooting results were obtained in T2, although the response obtained with A. rhizogenes didn’t differ from the T1 response. This appears to be an increasingly interesting methodology for adventitious rhizogenesis in this species.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.2 20092009-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019en10.4067/S0718-58392009000200019
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	rooting microshoots adult material
spellingShingle	rooting microshoots adult material Sánchez-Olate,Manuel Sáez,Patricia Ríos,Darcy Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
description	The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase of root induction was during 3 days in the dark while the second phase, root manifestation, was 27 days. Rooting percentage was evaluated and the induced root systems characterized (number, length, diameter, and root insertion zone) in all the procedures. The best rooting results were obtained in T2, although the response obtained with A. rhizogenes didn’t differ from the T1 response. This appears to be an increasingly interesting methodology for adventitious rhizogenesis in this species.
author	Sánchez-Olate,Manuel Sáez,Patricia Ríos,Darcy
author_facet	Sánchez-Olate,Manuel Sáez,Patricia Ríos,Darcy
author_sort	Sánchez-Olate,Manuel
title	Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
title_short	Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
title_full	Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
title_fullStr	Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
title_full_unstemmed	Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes
title_sort	rhizogenic induction in adult juglans regia l. cv. serr tissue induced by indole butyric acid and agrobacterium rhizogenes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019
work_keys_str_mv	AT sanchezolatemanuel rhizogenicinductioninadultjuglansregialcvserrtissueinducedbyindolebutyricacidandagrobacteriumrhizogenes AT saezpatricia rhizogenicinductioninadultjuglansregialcvserrtissueinducedbyindolebutyricacidandagrobacteriumrhizogenes AT riosdarcy rhizogenicinductioninadultjuglansregialcvserrtissueinducedbyindolebutyricacidandagrobacteriumrhizogenes
_version_	1714205270292299776

Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes

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