Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program

The Potato virus Y (PVY) is distributed worldwide and is one of the most damaging viruses in terms of yield reduction in the potato (Solanum tuberosum L.) crop with losses sometimes reaching 80%. To reduce its impact, there is great interest in obtaining varieties carrying the Ry adg gene that provi...

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Autores principales: Sagredo D,Boris, Mathias R,Mónica, Barrientos P,Claudia, Acuña B,Ivette, Kalazich B,Julio, Santos Rojas,José
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2009
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MAS
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spelling oai:scielo:S0718-583920090003000022018-10-01Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding ProgramSagredo D,BorisMathias R,MónicaBarrientos P,ClaudiaAcuña B,IvetteKalazich B,JulioSantos Rojas,José MAS extreme resistance The Potato virus Y (PVY) is distributed worldwide and is one of the most damaging viruses in terms of yield reduction in the potato (Solanum tuberosum L.) crop with losses sometimes reaching 80%. To reduce its impact, there is great interest in obtaining varieties carrying the Ry adg gene that provides extreme resistance to this virus. This could be facilitated with SCAR (sequence characterized amplified region) RYSC3 by molecular marker-assisted selection (MAS). This study compared the effectiveness of the RYSC3 marker in the detection of the Ry adg gene vs. biological tests on populations of the Potato Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA), Chile. Within the group of 71 progenitors, 30 plants had some kind of resistance to the virus, of which 17 were carriers of the RYSC3 marker. These genotypes came from Cornell University, Centro Internacional de la Papa (CIP), Peru, and INIA. The analysis of 460 progenies which came from three different crosses showed that 299 individuals amplified the RYSC3 marker and had the resistant phenotype, with the exception of one plant. Within the group of non-RYSC3 carrier plants, a significant percentage (22.5%) showed a resistant phenotype, indicating that these progenies segregate other R genes (e.g., hypersensitivity) that reduce biological test effectiveness. This high effectiveness (99.7%) in the detection of the Ry adg gene in both parents and segregating progenies, showed that this marker is appropriate in assisting selection of genotypes with extreme PVY-resistance in the potato breeding programs.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.3 20092009-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300002en10.4067/S0718-58392009000300002
institution Scielo Chile
collection Scielo Chile
language English
topic MAS
extreme resistance
spellingShingle MAS
extreme resistance
Sagredo D,Boris
Mathias R,Mónica
Barrientos P,Claudia
Acuña B,Ivette
Kalazich B,Julio
Santos Rojas,José
Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
description The Potato virus Y (PVY) is distributed worldwide and is one of the most damaging viruses in terms of yield reduction in the potato (Solanum tuberosum L.) crop with losses sometimes reaching 80%. To reduce its impact, there is great interest in obtaining varieties carrying the Ry adg gene that provides extreme resistance to this virus. This could be facilitated with SCAR (sequence characterized amplified region) RYSC3 by molecular marker-assisted selection (MAS). This study compared the effectiveness of the RYSC3 marker in the detection of the Ry adg gene vs. biological tests on populations of the Potato Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA), Chile. Within the group of 71 progenitors, 30 plants had some kind of resistance to the virus, of which 17 were carriers of the RYSC3 marker. These genotypes came from Cornell University, Centro Internacional de la Papa (CIP), Peru, and INIA. The analysis of 460 progenies which came from three different crosses showed that 299 individuals amplified the RYSC3 marker and had the resistant phenotype, with the exception of one plant. Within the group of non-RYSC3 carrier plants, a significant percentage (22.5%) showed a resistant phenotype, indicating that these progenies segregate other R genes (e.g., hypersensitivity) that reduce biological test effectiveness. This high effectiveness (99.7%) in the detection of the Ry adg gene in both parents and segregating progenies, showed that this marker is appropriate in assisting selection of genotypes with extreme PVY-resistance in the potato breeding programs.
author Sagredo D,Boris
Mathias R,Mónica
Barrientos P,Claudia
Acuña B,Ivette
Kalazich B,Julio
Santos Rojas,José
author_facet Sagredo D,Boris
Mathias R,Mónica
Barrientos P,Claudia
Acuña B,Ivette
Kalazich B,Julio
Santos Rojas,José
author_sort Sagredo D,Boris
title Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
title_short Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
title_full Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
title_fullStr Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
title_full_unstemmed Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program
title_sort evaluation of a scar rysc3 marker of the ry adg gene to select resistant genotypes to potato virus y (pvy) in the inia potato breeding program
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300002
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