Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination

A practical, rapid and inexpensive method for muscle glucose determination is proposed. The method is based on acid digestion of the sample, followed by neutralization of the solution and the subsequent glucose determination using a domestic glucometer for human blood glucose level control. In a pre...

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Autores principales:	Hargreaves B,Antonio, Barrales V,Luis, Barrales Z,Daniela, Riveros F,José Luis, Peña R,Iván
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	muscle glucose glycogen titration glycolytic potential
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300009
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spelling	oai:scielo:S0718-583920090003000092018-10-01Glycogen Determination in Bovine Muscle: A Proposal for Rapid DeterminationHargreaves B,AntonioBarrales V,LuisBarrales Z,DanielaRiveros F,José LuisPeña R,Iván muscle glucose glycogen titration glycolytic potential A practical, rapid and inexpensive method for muscle glucose determination is proposed. The method is based on acid digestion of the sample, followed by neutralization of the solution and the subsequent glucose determination using a domestic glucometer for human blood glucose level control. In a pre-experimental phase, the accuracy of this methodology and the titration method was determined, comparing results to pre-established glucose concentrations. There were significant differences between the glucose reading given by the glucometer and the pre-established glucose concentrations and the results given by the titration method. Nevertheless, when discrepancies (bias) were identified, differences resulted non-significant (P = 0.85). Subsequently, muscle glucose concentration (n = 24) was validated in the experimental phase and significant differences were shown between glucose concentration values given by the glucometer and the titration method, the latter considered as a standard method or as a reference (P < 0.0001). Once the sources of discrepancies were identified and removed, the differences observed between the values given by the two methods were not statistically significant (P = 0.99). It was concluded that the glucometer is a potential method for muscle glucose determination, but must be improved.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.3 20092009-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300009en10.4067/S0718-58392009000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	muscle glucose glycogen titration glycolytic potential
spellingShingle	muscle glucose glycogen titration glycolytic potential Hargreaves B,Antonio Barrales V,Luis Barrales Z,Daniela Riveros F,José Luis Peña R,Iván Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
description	A practical, rapid and inexpensive method for muscle glucose determination is proposed. The method is based on acid digestion of the sample, followed by neutralization of the solution and the subsequent glucose determination using a domestic glucometer for human blood glucose level control. In a pre-experimental phase, the accuracy of this methodology and the titration method was determined, comparing results to pre-established glucose concentrations. There were significant differences between the glucose reading given by the glucometer and the pre-established glucose concentrations and the results given by the titration method. Nevertheless, when discrepancies (bias) were identified, differences resulted non-significant (P = 0.85). Subsequently, muscle glucose concentration (n = 24) was validated in the experimental phase and significant differences were shown between glucose concentration values given by the glucometer and the titration method, the latter considered as a standard method or as a reference (P < 0.0001). Once the sources of discrepancies were identified and removed, the differences observed between the values given by the two methods were not statistically significant (P = 0.99). It was concluded that the glucometer is a potential method for muscle glucose determination, but must be improved.
author	Hargreaves B,Antonio Barrales V,Luis Barrales Z,Daniela Riveros F,José Luis Peña R,Iván
author_facet	Hargreaves B,Antonio Barrales V,Luis Barrales Z,Daniela Riveros F,José Luis Peña R,Iván
author_sort	Hargreaves B,Antonio
title	Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
title_short	Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
title_full	Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
title_fullStr	Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
title_full_unstemmed	Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination
title_sort	glycogen determination in bovine muscle: a proposal for rapid determination
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300009
work_keys_str_mv	AT hargreavesbantonio glycogendeterminationinbovinemuscleaproposalforrapiddetermination AT barralesvluis glycogendeterminationinbovinemuscleaproposalforrapiddetermination AT barraleszdaniela glycogendeterminationinbovinemuscleaproposalforrapiddetermination AT riverosfjoseluis glycogendeterminationinbovinemuscleaproposalforrapiddetermination AT penarivan glycogendeterminationinbovinemuscleaproposalforrapiddetermination
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Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination

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