Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)

Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pas...

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Autores principales:	Moyano A,Stella, Lanuza,Francisco, Torres B,Alfredo, Cisternas A,Ernesto, Fuentes V,Marcela
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	mycotoxin neurotoxin endophyte fungi
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019
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spelling	oai:scielo:S0718-583920090003000192018-10-01Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)Moyano A,StellaLanuza,FranciscoTorres B,AlfredoCisternas A,ErnestoFuentes V,Marcela mycotoxin neurotoxin endophyte fungi Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pasture in southern Chile, it is necessary to know the content of lolitrem-B to optimize grazing management and reduce the effect on animal health. The objective of this study was to implement a method to determine lolitrem-B in ryegrass. Ryegrass samples were used without the endophyte, freeze-dried, and kept in darkness at -18 °C to establish the method. Then, a pure lolitrem-B standard was added and the toxin was extracted with a mixture of chloroform-methanol (2:1), purified in manually prepared silica gel 60 columns. Five standard addition levels were studied (0.05, 0.10, 0.30, 0.60, and 1.00 mg kg-1) with five replicates per level. The recovery was 96.6 to 99.9%, on average and the coefficient of variation (CV) ranged between 0.9 and 5.9%. Quantification was done by high-performance liquid chromatography (HPLC) and fluorescence detection. The quantification limit was 0.05 mg kg-1. This method of determining neurotoxin lolitrem-B in ryegrass samples was implemented for the first time in Chile. It is fast and inexpensive; it has good precision, accuracy and repeatability.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.3 20092009-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019en10.4067/S0718-58392009000300019
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mycotoxin neurotoxin endophyte fungi
spellingShingle	mycotoxin neurotoxin endophyte fungi Moyano A,Stella Lanuza,Francisco Torres B,Alfredo Cisternas A,Ernesto Fuentes V,Marcela Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
description	Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pasture in southern Chile, it is necessary to know the content of lolitrem-B to optimize grazing management and reduce the effect on animal health. The objective of this study was to implement a method to determine lolitrem-B in ryegrass. Ryegrass samples were used without the endophyte, freeze-dried, and kept in darkness at -18 °C to establish the method. Then, a pure lolitrem-B standard was added and the toxin was extracted with a mixture of chloroform-methanol (2:1), purified in manually prepared silica gel 60 columns. Five standard addition levels were studied (0.05, 0.10, 0.30, 0.60, and 1.00 mg kg-1) with five replicates per level. The recovery was 96.6 to 99.9%, on average and the coefficient of variation (CV) ranged between 0.9 and 5.9%. Quantification was done by high-performance liquid chromatography (HPLC) and fluorescence detection. The quantification limit was 0.05 mg kg-1. This method of determining neurotoxin lolitrem-B in ryegrass samples was implemented for the first time in Chile. It is fast and inexpensive; it has good precision, accuracy and repeatability.
author	Moyano A,Stella Lanuza,Francisco Torres B,Alfredo Cisternas A,Ernesto Fuentes V,Marcela
author_facet	Moyano A,Stella Lanuza,Francisco Torres B,Alfredo Cisternas A,Ernesto Fuentes V,Marcela
author_sort	Moyano A,Stella
title	Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
title_short	Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
title_full	Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
title_fullStr	Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
title_full_unstemmed	Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
title_sort	implementation of a method to determine lolitrem-b in ryegrass (lolium perenne l.) by liquid chromatography (hplc)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019
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Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)

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