Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 c...

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Autores principales:	Avilés,Fabiola, Ríos,Darcy, González,Ricardo, Sánchez-Olate,Manuel
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	auxins citoquinines walnut callus leaves
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300020
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spelling	oai:scielo:S0718-583920090003000202018-10-01Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)Avilés,FabiolaRíos,DarcyGonzález,RicardoSánchez-Olate,Manuel auxins citoquinines walnut callus leaves To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm², previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.3 20092009-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300020en10.4067/S0718-58392009000300020
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	auxins citoquinines walnut callus leaves
spellingShingle	auxins citoquinines walnut callus leaves Avilés,Fabiola Ríos,Darcy González,Ricardo Sánchez-Olate,Manuel Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
description	To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm², previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.
author	Avilés,Fabiola Ríos,Darcy González,Ricardo Sánchez-Olate,Manuel
author_facet	Avilés,Fabiola Ríos,Darcy González,Ricardo Sánchez-Olate,Manuel
author_sort	Avilés,Fabiola
title	Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
title_short	Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
title_full	Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
title_fullStr	Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
title_full_unstemmed	Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
title_sort	effect of culture medium in callogenesis from adult walnut leaves (juglans regia l.)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300020
work_keys_str_mv	AT avilesfabiola effectofculturemediumincallogenesisfromadultwalnutleavesjuglansregial AT riosdarcy effectofculturemediumincallogenesisfromadultwalnutleavesjuglansregial AT gonzalezricardo effectofculturemediumincallogenesisfromadultwalnutleavesjuglansregial AT sanchezolatemanuel effectofculturemediumincallogenesisfromadultwalnutleavesjuglansregial
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Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

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