Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid

The Macrophomina phaseolina (Tassi) Goid. fungus, causal agent of charcoal root rot, is considered one of the most important pathogens in forest nurseries. Recent studies in Chile have verified the antagonistic capacity of diverse bacterial isolates against M. phaseolina in vitro as well as in field...

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Autores principales:	Gacitúa A,Sandra, Valiente F,Carolina, Díaz P,Katy, Hernández C,José, Uribe M,Matilde, Sanfuentes V,Eugenio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	biocontrol forest nursery
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400007
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spelling	oai:scielo:S0718-583920090004000072018-10-01Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) GoidGacitúa A,SandraValiente F,CarolinaDíaz P,KatyHernández C,JoséUribe M,MatildeSanfuentes V,Eugenio biocontrol forest nursery The Macrophomina phaseolina (Tassi) Goid. fungus, causal agent of charcoal root rot, is considered one of the most important pathogens in forest nurseries. Recent studies in Chile have verified the antagonistic capacity of diverse bacterial isolates against M. phaseolina in vitro as well as in field testing. The objectives of this study were to identify Bacillus isolates and establish the mechanisms of antagonism against M. phaseolina. Isolates were identified with the bioMérieux biochemical kit. Tests were done in vitro and consisted in determining the production of volatile metabolites, siderophores, and the effect of different culture temperatures (20, 25, 30, and 35 °C) on the production of diffusible metabolites and inhibition of mycelial growth of the pathogen. The isolates were identified as Bacillus subtilis IX 007, B. amyloliquefaciens VII 015 and VIII 016, B. pumilus IX 030, and B. stearothermophilus TM 008. It was not possible to identify the (-) Gram VI 009 isolate. At all the evaluated temperatures, isolates inhibited M. phaseolina, reaching 75% reduction of mycelial growth (B. subtilis IX 007 at 25 °C). Some isolates showed volatile metabolites and siderophore production as possible mechanisms of antagonism.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400007en10.4067/S0718-58392009000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biocontrol forest nursery
spellingShingle	biocontrol forest nursery Gacitúa A,Sandra Valiente F,Carolina Díaz P,Katy Hernández C,José Uribe M,Matilde Sanfuentes V,Eugenio Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
description	The Macrophomina phaseolina (Tassi) Goid. fungus, causal agent of charcoal root rot, is considered one of the most important pathogens in forest nurseries. Recent studies in Chile have verified the antagonistic capacity of diverse bacterial isolates against M. phaseolina in vitro as well as in field testing. The objectives of this study were to identify Bacillus isolates and establish the mechanisms of antagonism against M. phaseolina. Isolates were identified with the bioMérieux biochemical kit. Tests were done in vitro and consisted in determining the production of volatile metabolites, siderophores, and the effect of different culture temperatures (20, 25, 30, and 35 °C) on the production of diffusible metabolites and inhibition of mycelial growth of the pathogen. The isolates were identified as Bacillus subtilis IX 007, B. amyloliquefaciens VII 015 and VIII 016, B. pumilus IX 030, and B. stearothermophilus TM 008. It was not possible to identify the (-) Gram VI 009 isolate. At all the evaluated temperatures, isolates inhibited M. phaseolina, reaching 75% reduction of mycelial growth (B. subtilis IX 007 at 25 °C). Some isolates showed volatile metabolites and siderophore production as possible mechanisms of antagonism.
author	Gacitúa A,Sandra Valiente F,Carolina Díaz P,Katy Hernández C,José Uribe M,Matilde Sanfuentes V,Eugenio
author_facet	Gacitúa A,Sandra Valiente F,Carolina Díaz P,Katy Hernández C,José Uribe M,Matilde Sanfuentes V,Eugenio
author_sort	Gacitúa A,Sandra
title	Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
title_short	Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
title_full	Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
title_fullStr	Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
title_full_unstemmed	Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid
title_sort	identification and biological characterization of isolates with activity inhibitive against macrophomina phaseolina (tassi) goid
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400007
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_version_	1714205275243675648

Identification and Biological Characterization of Isolates with Activity Inhibitive against Macrophomina phaseolina (Tassi) Goid

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