Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 °C) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations wer...

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Autores principales:	Rodríguez,Marta, Gerding,Marcos, France,Andrés, Ceballos,Ricardo
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	biological control entomopathogenic fungi varroasis insect pathology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400009
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spelling	oai:scielo:S0718-583920090004000092018-10-01Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field TrialsRodríguez,MartaGerding,MarcosFrance,AndrésCeballos,Ricardo biological control entomopathogenic fungi varroasis insect pathology The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 °C) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations were carried out by spraying and increasing the concentration from zero to 10(8) conidia mL-1 on varroa adults. Lethal concentrations required for 50 and 90% mite mortality (LC50 and LC90) were 3.8 x 10(5) and 8 x 10(7) conidia mL-1, respectively (χ2 = 2.03). In the autumn field trials, three application methods (doses of 5 x 10(10) conidia per hive) were evaluated. The treatments were: a) conidia stamped on filter paper, located on every second frame inside the hive; b) dry conidia sprinkled on and between frames; and c) dry conidia in a dispenser path at the entrance of the hive. Furthermore, untreated hives were included as controls. After 21 days of treatment, the dry conidia sprinkled on and between frames showed 67% less bees infested by the mite than the control (p < 0.05). Average bee mortality observed was significantly different between treatments (p < 0.05). The sprinkled dry conidia caused the highest mortality of bees. In spring, this treatment produced increased mite mortality and without bee mortality. These results demonstrated that it is possible to develop a biological insecticide based on this isolate to generate an alternative to control V. destructor.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400009en10.4067/S0718-58392009000400009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biological control entomopathogenic fungi varroasis insect pathology
spellingShingle	biological control entomopathogenic fungi varroasis insect pathology Rodríguez,Marta Gerding,Marcos France,Andrés Ceballos,Ricardo Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
description	The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 °C) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations were carried out by spraying and increasing the concentration from zero to 10(8) conidia mL-1 on varroa adults. Lethal concentrations required for 50 and 90% mite mortality (LC50 and LC90) were 3.8 x 10(5) and 8 x 10(7) conidia mL-1, respectively (χ2 = 2.03). In the autumn field trials, three application methods (doses of 5 x 10(10) conidia per hive) were evaluated. The treatments were: a) conidia stamped on filter paper, located on every second frame inside the hive; b) dry conidia sprinkled on and between frames; and c) dry conidia in a dispenser path at the entrance of the hive. Furthermore, untreated hives were included as controls. After 21 days of treatment, the dry conidia sprinkled on and between frames showed 67% less bees infested by the mite than the control (p < 0.05). Average bee mortality observed was significantly different between treatments (p < 0.05). The sprinkled dry conidia caused the highest mortality of bees. In spring, this treatment produced increased mite mortality and without bee mortality. These results demonstrated that it is possible to develop a biological insecticide based on this isolate to generate an alternative to control V. destructor.
author	Rodríguez,Marta Gerding,Marcos France,Andrés Ceballos,Ricardo
author_facet	Rodríguez,Marta Gerding,Marcos France,Andrés Ceballos,Ricardo
author_sort	Rodríguez,Marta
title	Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
title_short	Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
title_full	Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
title_fullStr	Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
title_full_unstemmed	Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials
title_sort	evaluation of metarhizium anisopliae var. anisopliae qu-m845 isolate to control varroa destructor (acari: varroidae) in laboratory and field trials
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400009
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Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

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