Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and pres...

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Autores principales:	Muñoz,Manuel, Seemann,Peter, Jara,Gloria, Riegel,Ricardo
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2009
Materias:	TIS liquid culture micropropagation acclimatization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014
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spelling	oai:scielo:S0718-583920090004000142018-10-01Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala speciesMuñoz,ManuelSeemann,PeterJara,GloriaRiegel,Ricardo TIS liquid culture micropropagation acclimatization Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared liquid and solid media, and in the third experiment, a temporary immersion system (TIS), and conventional culture in static liquid, shaken liquid and gelled Murashige and Skoog (MS) media were compared. By using larger (350 mL) flasks with higher (50 mL) media volume, 100% more fresh weight of microbulb was obtained that treatment with smaller flasks (45 mL) and media volume (10 mL). In gelled medium, hyperhydricity affected only 5% of explants, while in liquid medium was 16-40%. Survival to acclimatization reached 87-94% for plants from gelled medium; from liquid medium only 38-69%. TIS yielded higher propagation rate (1.9 shoots in 30 d) compared with shaken liquid medium (1.0) (P < 0.05) in R. ananuca only. Current procedures are appropriate for the support of ex situ conservation and germplasm bank establishment.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.69 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014en10.4067/S0718-58392009000400014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	TIS liquid culture micropropagation acclimatization
spellingShingle	TIS liquid culture micropropagation acclimatization Muñoz,Manuel Seemann,Peter Jara,Gloria Riegel,Ricardo Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
description	Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared liquid and solid media, and in the third experiment, a temporary immersion system (TIS), and conventional culture in static liquid, shaken liquid and gelled Murashige and Skoog (MS) media were compared. By using larger (350 mL) flasks with higher (50 mL) media volume, 100% more fresh weight of microbulb was obtained that treatment with smaller flasks (45 mL) and media volume (10 mL). In gelled medium, hyperhydricity affected only 5% of explants, while in liquid medium was 16-40%. Survival to acclimatization reached 87-94% for plants from gelled medium; from liquid medium only 38-69%. TIS yielded higher propagation rate (1.9 shoots in 30 d) compared with shaken liquid medium (1.0) (P < 0.05) in R. ananuca only. Current procedures are appropriate for the support of ex situ conservation and germplasm bank establishment.
author	Muñoz,Manuel Seemann,Peter Jara,Gloria Riegel,Ricardo
author_facet	Muñoz,Manuel Seemann,Peter Jara,Gloria Riegel,Ricardo
author_sort	Muñoz,Manuel
title	Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
title_short	Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
title_full	Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
title_fullStr	Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
title_full_unstemmed	Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species
title_sort	influence of vessel type, physical state of medium and temporary immersion on the micropropagation of three rhodophiala species
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014
work_keys_str_mv	AT munozmanuel influenceofvesseltypephysicalstateofmediumandtemporaryimmersiononthemicropropagationofthreerhodophialaspecies AT seemannpeter influenceofvesseltypephysicalstateofmediumandtemporaryimmersiononthemicropropagationofthreerhodophialaspecies AT jaragloria influenceofvesseltypephysicalstateofmediumandtemporaryimmersiononthemicropropagationofthreerhodophialaspecies AT riegelricardo influenceofvesseltypephysicalstateofmediumandtemporaryimmersiononthemicropropagationofthreerhodophialaspecies
_version_	1714205276435906560

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

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