Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis

We have developed a parallel, rapid, high-throughput oligonucleotide microarray-based assay for the reliable detection and genotyping of three cry genes (cry1, cry2 and cry9) in Bacillus thuringiensis (Bt). After the non-polymerase chain reaction (PCR), amplified Bt genomic DNA were fluorescent-labe...

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Autores principales:	Xu-Guang,Liu, Fu-Ping,Song, Si-Yuan,Wen, Sheng-Qi,Wang, Da-Fang,Huang, Jie,Zhang
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2010
Materias:	oligonucleotide microarray cry genes genotyping random primer labeling bioinformatics
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200004
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spelling	oai:scielo:S0718-583920100002000042018-10-01Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensisXu-Guang,LiuFu-Ping,SongSi-Yuan,WenSheng-Qi,WangDa-Fang,HuangJie,Zhang oligonucleotide microarray cry genes genotyping random primer labeling bioinformatics We have developed a parallel, rapid, high-throughput oligonucleotide microarray-based assay for the reliable detection and genotyping of three cry genes (cry1, cry2 and cry9) in Bacillus thuringiensis (Bt). After the non-polymerase chain reaction (PCR), amplified Bt genomic DNA were fluorescent-labeled using a random primer. The corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid for the detection and genotyping of genes. This type of assay is also a potentially valuable tool for identification and characterization of bacterial functional genes in general.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.70 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200004en10.4067/S0718-58392010000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	oligonucleotide microarray cry genes genotyping random primer labeling bioinformatics
spellingShingle	oligonucleotide microarray cry genes genotyping random primer labeling bioinformatics Xu-Guang,Liu Fu-Ping,Song Si-Yuan,Wen Sheng-Qi,Wang Da-Fang,Huang Jie,Zhang Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
description	We have developed a parallel, rapid, high-throughput oligonucleotide microarray-based assay for the reliable detection and genotyping of three cry genes (cry1, cry2 and cry9) in Bacillus thuringiensis (Bt). After the non-polymerase chain reaction (PCR), amplified Bt genomic DNA were fluorescent-labeled using a random primer. The corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid for the detection and genotyping of genes. This type of assay is also a potentially valuable tool for identification and characterization of bacterial functional genes in general.
author	Xu-Guang,Liu Fu-Ping,Song Si-Yuan,Wen Sheng-Qi,Wang Da-Fang,Huang Jie,Zhang
author_facet	Xu-Guang,Liu Fu-Ping,Song Si-Yuan,Wen Sheng-Qi,Wang Da-Fang,Huang Jie,Zhang
author_sort	Xu-Guang,Liu
title	Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
title_short	Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
title_full	Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
title_fullStr	Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
title_full_unstemmed	Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis
title_sort	application of oligonucleotide microarray for the detection and genotyping of cry genes in bacillu thuringiensis
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200004
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Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis

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