Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting

The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance o...

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Autores principales:	Wong,Wendy, Barba,Paola, Álvarez,Catalina, Castro,Álvaro, Acuña,Manuel, Zamora,Pablo, Rosales,Marlene, Dell ’Orto,Paola, Moynihan,Michael R, Scorza,Ralph, Prieto,Humberto
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2010
Materias:	C5 Chilean isolates micro-grafting
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000300004
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spelling	oai:scielo:S0718-583920100003000042018-10-01Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-GraftingWong,WendyBarba,PaolaÁlvarez,CatalinaCastro,ÁlvaroAcuña,ManuelZamora,PabloRosales,MarleneDell ’Orto,PaolaMoynihan,Michael RScorza,RalphPrieto,Humberto C5 Chilean isolates micro-grafting The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance of C5 to several D and M strain PPV isolates. In Chile, the quarantine regulations for PPV and for genetically modified (GM) plants require that the testing of C5 for resistance to Chilean PPV isolates be done under controlled isolated conditions. To carry out these tests C5 shoots were multiplied in vitro and micro-grafted onto four Adesoto101 (Prunus insititia L.) rootstock populations that had been previously infected each with one of four Chilean PPV-Ds. Tests were carried out under controlled conditions in a biosafety greenhouse. Symptoms appearance, virus detection, and viral mRNA levels for the cylindrical inclusion (CI) and CP genes were determined during three consecutive growing seasons. Complete resistance to all PPV isolates was demonstrated during the first 2 yr in all of the C5 plants. In the third season, four of 10 C5 plants showed mild symptoms on leaves close to the graft union and low but detectable CI mRNA levels in the C5 scions. These results support the effectiveness of using of micro-grafting on P. insititia for PPV resistance studies, especially in the limited space of a quarantine facility; whereas resistance levels in C5 after 3 yr indicate the importance of long term and field scale evaluations.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.70 n.3 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000300004en10.4067/S0718-58392010000300004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	C5 Chilean isolates micro-grafting
spellingShingle	C5 Chilean isolates micro-grafting Wong,Wendy Barba,Paola Álvarez,Catalina Castro,Álvaro Acuña,Manuel Zamora,Pablo Rosales,Marlene Dell ’Orto,Paola Moynihan,Michael R Scorza,Ralph Prieto,Humberto Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
description	The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance of C5 to several D and M strain PPV isolates. In Chile, the quarantine regulations for PPV and for genetically modified (GM) plants require that the testing of C5 for resistance to Chilean PPV isolates be done under controlled isolated conditions. To carry out these tests C5 shoots were multiplied in vitro and micro-grafted onto four Adesoto101 (Prunus insititia L.) rootstock populations that had been previously infected each with one of four Chilean PPV-Ds. Tests were carried out under controlled conditions in a biosafety greenhouse. Symptoms appearance, virus detection, and viral mRNA levels for the cylindrical inclusion (CI) and CP genes were determined during three consecutive growing seasons. Complete resistance to all PPV isolates was demonstrated during the first 2 yr in all of the C5 plants. In the third season, four of 10 C5 plants showed mild symptoms on leaves close to the graft union and low but detectable CI mRNA levels in the C5 scions. These results support the effectiveness of using of micro-grafting on P. insititia for PPV resistance studies, especially in the limited space of a quarantine facility; whereas resistance levels in C5 after 3 yr indicate the importance of long term and field scale evaluations.
author	Wong,Wendy Barba,Paola Álvarez,Catalina Castro,Álvaro Acuña,Manuel Zamora,Pablo Rosales,Marlene Dell ’Orto,Paola Moynihan,Michael R Scorza,Ralph Prieto,Humberto
author_facet	Wong,Wendy Barba,Paola Álvarez,Catalina Castro,Álvaro Acuña,Manuel Zamora,Pablo Rosales,Marlene Dell ’Orto,Paola Moynihan,Michael R Scorza,Ralph Prieto,Humberto
author_sort	Wong,Wendy
title	Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
title_short	Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
title_full	Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
title_fullStr	Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
title_full_unstemmed	Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting
title_sort	evaluation of the resistance of transgenic c5 plum (prunus domestica l.) against four chilean plum pox virus isolates through micro-grafting
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000300004
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Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting

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