Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)

Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current prop...

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Autores principales:	Garriga Caraballo,Miguel, González Oramas,Gerardo, Alemán García,Silvia, Abreu Cruz,Enildo, Quiroz Bravo,Karla, D.S. Caligari,Peter, García-González,Rolando
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2010
Materias:	propagation thidiazuron 6-benzylamynopurine in vitro culture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400003
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spelling	oai:scielo:S0718-583920100004000032018-10-01Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)Garriga Caraballo,MiguelGonzález Oramas,GerardoAlemán García,SilviaAbreu Cruz,EnildoQuiroz Bravo,KarlaD.S. Caligari,PeterGarcía-González,Rolando propagation thidiazuron 6-benzylamynopurine in vitro culture Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current propagation technologies are not satisfying the grower’s demands. A. fourcroydes has been cultivated n vitro but it has shown some recalcitrant behaviour during the multiplication and rooting stages. The combination of 6-benzylamynopurine (BAP) (0.75 mg L-1) and indolebutyric acid (IBA) (1.0 mg L-1) instead of 2,4-dichlorophenoxyacetic acid (2,4-D) significantly improved explant survival and shooting during the establishment of n vitro young shoots. Combining thidiazuron (TDZ) (0.5 or 0.75 mg L-1) with BAP (1.0 mg L-1) and IBA (1.0 mg L-1) in the basal medium increased the multiplication rate of henequen and significantly speeded out bud dormancy breaking. To improve rooting of the micropropagated shoots, the addition of IBA and naphthylacetic acid (NAA) was tested. The best rooting efficiency was obtained when the basal medium was supplemented with 0.5 or 0.75 mg L-1 of NAA, giving 100% of rooted explants and an average of 9.40 and 11.55 roots per explants, respectively. Over 94% of micropropagated plants survived the ex vitro weaning step and no morphological disorders were observed in any of the plants. Modification of plant growth regulators composition in the medium was a key factor to improve the efficiency of the micropropagation technology of henequen.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.70 n.4 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400003en10.4067/S0718-58392010000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	propagation thidiazuron 6-benzylamynopurine in vitro culture
spellingShingle	propagation thidiazuron 6-benzylamynopurine in vitro culture Garriga Caraballo,Miguel González Oramas,Gerardo Alemán García,Silvia Abreu Cruz,Enildo Quiroz Bravo,Karla D.S. Caligari,Peter García-González,Rolando Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
description	Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current propagation technologies are not satisfying the grower’s demands. A. fourcroydes has been cultivated n vitro but it has shown some recalcitrant behaviour during the multiplication and rooting stages. The combination of 6-benzylamynopurine (BAP) (0.75 mg L-1) and indolebutyric acid (IBA) (1.0 mg L-1) instead of 2,4-dichlorophenoxyacetic acid (2,4-D) significantly improved explant survival and shooting during the establishment of n vitro young shoots. Combining thidiazuron (TDZ) (0.5 or 0.75 mg L-1) with BAP (1.0 mg L-1) and IBA (1.0 mg L-1) in the basal medium increased the multiplication rate of henequen and significantly speeded out bud dormancy breaking. To improve rooting of the micropropagated shoots, the addition of IBA and naphthylacetic acid (NAA) was tested. The best rooting efficiency was obtained when the basal medium was supplemented with 0.5 or 0.75 mg L-1 of NAA, giving 100% of rooted explants and an average of 9.40 and 11.55 roots per explants, respectively. Over 94% of micropropagated plants survived the ex vitro weaning step and no morphological disorders were observed in any of the plants. Modification of plant growth regulators composition in the medium was a key factor to improve the efficiency of the micropropagation technology of henequen.
author	Garriga Caraballo,Miguel González Oramas,Gerardo Alemán García,Silvia Abreu Cruz,Enildo Quiroz Bravo,Karla D.S. Caligari,Peter García-González,Rolando
author_facet	Garriga Caraballo,Miguel González Oramas,Gerardo Alemán García,Silvia Abreu Cruz,Enildo Quiroz Bravo,Karla D.S. Caligari,Peter García-González,Rolando
author_sort	Garriga Caraballo,Miguel
title	Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
title_short	Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
title_full	Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
title_fullStr	Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
title_full_unstemmed	Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
title_sort	management of auxin-cytokinin interactions to improve micropropagation protocol of henequen (agave fourcroydes lem.)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400003
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Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)

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