Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Araya,Carolina, Peña,Elizabeth, Salazar,Erika, Román,Lisset, Medina,Claudia, Mora,Roxana, Aljaro,Agustín, Rosales,Inés-Marlene
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2011
Materias:	Lettuce big-vein disease symptom expression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392011000100008
record_format	dspace
spelling	oai:scielo:S0718-583920110001000082018-10-01Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein diseaseAraya,CarolinaPeña,ElizabethSalazar,ErikaRomán,LissetMedina,ClaudiaMora,RoxanaAljaro,AgustínRosales,Inés-Marlene Lettuce big-vein disease symptom expression Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat protein accumulation did not differ for the two viruses associated with this disease among lettuce plants showing different symptom severity. Similarly, abundance of Mirafiori lettuce big-vein virus (MLBVV)-RNA3 or Lettuce big-vein associated virus (LBVaV)-RNA-2 were not different (P > 0.05) for diverse big vein disease severity rating scales. This suggests that symptom severity expressed by big-vein diseased lettuce plants did not necessarily reflect the accumulation of viruses associated with this disease in the host. Therefore, lettuce plants showing mild symptoms of BVD do not necessarily present lower virus levels than plants showing more severe symptomatology.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.71 n.1 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008en10.4067/S0718-58392011000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lettuce big-vein disease symptom expression
spellingShingle	Lettuce big-vein disease symptom expression Araya,Carolina Peña,Elizabeth Salazar,Erika Román,Lisset Medina,Claudia Mora,Roxana Aljaro,Agustín Rosales,Inés-Marlene Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
description	Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat protein accumulation did not differ for the two viruses associated with this disease among lettuce plants showing different symptom severity. Similarly, abundance of Mirafiori lettuce big-vein virus (MLBVV)-RNA3 or Lettuce big-vein associated virus (LBVaV)-RNA-2 were not different (P > 0.05) for diverse big vein disease severity rating scales. This suggests that symptom severity expressed by big-vein diseased lettuce plants did not necessarily reflect the accumulation of viruses associated with this disease in the host. Therefore, lettuce plants showing mild symptoms of BVD do not necessarily present lower virus levels than plants showing more severe symptomatology.
author	Araya,Carolina Peña,Elizabeth Salazar,Erika Román,Lisset Medina,Claudia Mora,Roxana Aljaro,Agustín Rosales,Inés-Marlene
author_facet	Araya,Carolina Peña,Elizabeth Salazar,Erika Román,Lisset Medina,Claudia Mora,Roxana Aljaro,Agustín Rosales,Inés-Marlene
author_sort	Araya,Carolina
title	Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
title_short	Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
title_full	Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
title_fullStr	Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
title_full_unstemmed	Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease
title_sort	symptom severity and viral protein or rna accumulation in lettuce affected by big-vein disease
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008
work_keys_str_mv	AT arayacarolina symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT penaelizabeth symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT salazarerika symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT romanlisset symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT medinaclaudia symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT moraroxana symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT aljaroagustin symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease AT rosalesinesmarlene symptomseverityandviralproteinorrnaaccumulationinlettuceaffectedbybigveindisease
_version_	1714205291947491328

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Ejemplares similares