Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method

Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Barchiesi-Ferrari,Claudia, Alomar,Daniel, Miranda,Horacio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2011
Materias:	Cellulase incubation enzymatic digestibility in vitro digestibility
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392011000200010
record_format	dspace
spelling	oai:scielo:S0718-583920110002000102018-10-01Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic MethodBarchiesi-Ferrari,ClaudiaAlomar,DanielMiranda,Horacio Cellulase incubation enzymatic digestibility in vitro digestibility Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 and 6.25 g L-1), incubation time (24 or 48 h) and type of final washing of the residue (water or acetone) on the in vitro digestibility of the dry matter (DMD), organic matter (OMD) and content of digestible organic matter in the DM (D value) of silages made at three maturity stages from three types of pastures: a) permanent pasture (Dactylis glomerata, Lolium perenne, Bromus catharticus Vahl var. catharticus, Trifolium repens and Holcus lanatus); b) Italian ryegrass ley (Lolium multiflorum Lam. cv. Tama); c) oats (Avena sativa L.) and d) mixed pasture (L. perenne-T. repens). Regression equations among cellulase results and in vitro values obtained with rumen fluid were also developed. Higher enzyme concentration, longer incubation time and final washing with acetone, resulted in a significant (P < 0.05) increase in DMD, OMD and D value. A strong interaction (P < 0.001) among forage type, maturity stage and incubation time was observed. Interactions between stage and enzyme concentration were also apparent (P < 0.05) for D value, DMD and OMD. Type of pasture and maturity affected the performance of the enzymatic method to predict in vitro digestibility obtained with rumen fluid. Therefore, different prediction equations should be developed for any combination of the above mentioned factors. Adjusting this methodology with in vivo data is encouraged to improve prediction of digestibility values.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.71 n.2 20112011-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200010en10.4067/S0718-58392011000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cellulase incubation enzymatic digestibility in vitro digestibility
spellingShingle	Cellulase incubation enzymatic digestibility in vitro digestibility Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
description	Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 and 6.25 g L-1), incubation time (24 or 48 h) and type of final washing of the residue (water or acetone) on the in vitro digestibility of the dry matter (DMD), organic matter (OMD) and content of digestible organic matter in the DM (D value) of silages made at three maturity stages from three types of pastures: a) permanent pasture (Dactylis glomerata, Lolium perenne, Bromus catharticus Vahl var. catharticus, Trifolium repens and Holcus lanatus); b) Italian ryegrass ley (Lolium multiflorum Lam. cv. Tama); c) oats (Avena sativa L.) and d) mixed pasture (L. perenne-T. repens). Regression equations among cellulase results and in vitro values obtained with rumen fluid were also developed. Higher enzyme concentration, longer incubation time and final washing with acetone, resulted in a significant (P < 0.05) increase in DMD, OMD and D value. A strong interaction (P < 0.001) among forage type, maturity stage and incubation time was observed. Interactions between stage and enzyme concentration were also apparent (P < 0.05) for D value, DMD and OMD. Type of pasture and maturity affected the performance of the enzymatic method to predict in vitro digestibility obtained with rumen fluid. Therefore, different prediction equations should be developed for any combination of the above mentioned factors. Adjusting this methodology with in vivo data is encouraged to improve prediction of digestibility values.
author	Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio
author_facet	Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio
author_sort	Barchiesi-Ferrari,Claudia
title	Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
title_short	Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
title_full	Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
title_fullStr	Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
title_full_unstemmed	Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method
title_sort	pepsin-cellulase digestibility of pasture silages: effects of pasture type, maturity stage, and variations in the enzymatic method
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200010
work_keys_str_mv	AT barchiesiferrariclaudia pepsincellulasedigestibilityofpasturesilageseffectsofpasturetypematuritystageandvariationsintheenzymaticmethod AT alomardaniel pepsincellulasedigestibilityofpasturesilageseffectsofpasturetypematuritystageandvariationsintheenzymaticmethod AT mirandahoracio pepsincellulasedigestibilityofpasturesilageseffectsofpasturetypematuritystageandvariationsintheenzymaticmethod
_version_	1714205295569272832

Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method

Ejemplares similares