Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages

In pasture-based dairy herds where silage is a widely adopted supplement, optimized feeding requires reliable estimations of nutritional quality of this conserved forage. Metabolizable energy, an important nutritional fraction, can be predicted from digestibility-related traits, such as the digestib...

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Autores principales:	Barchiesi-Ferrari,Claudia, Alomar,Daniel, Miranda,Horacio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2011
Materias:	pasture silage digestibility of dry matter
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200011
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spelling	oai:scielo:S0718-583920110002000112018-10-01Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture SilagesBarchiesi-Ferrari,ClaudiaAlomar,DanielMiranda,Horacio pasture silage digestibility of dry matter In pasture-based dairy herds where silage is a widely adopted supplement, optimized feeding requires reliable estimations of nutritional quality of this conserved forage. Metabolizable energy, an important nutritional fraction, can be predicted from digestibility-related traits, such as the digestible organic matter contained in the dry matter (D-value). The aim of the present study was to evaluate the prediction of D-value and dry matter digestibility (DMD) of grass silages made from four different pastures and maturity stages, using the pepsin-cellulase method. Fungal cellulase was used, applying different enzyme concentrations, incubation times and types of final wash. The silages were prepared from permanent pasture (Dactylis glomerata L., Lolium perenne L., Bromus catharticus Vahl var. catharticus, Trifolium repens L. and Holcus lanatus L.), rotation pasture (Lolium multiflorum Lam. cv. Tama), oats (Avena sativa L.), and mixed pasture (L. perenne-T. repens). These were harvested at three different physiological stages (vegetative, ear emergence and dough grain). The treatment using an incubation time of 24 h, a cellulase concentration of 6.25 g L-1 and final wash with water (Treatment 3) presented the best prediction capacity of the in vivo D-value (R² = 0.78) and in vivo DMD (R² = 0.71). In vivo D-value prediction improved (R² = 0.8) when a chemical determination (crude fibre, gross energy, neutral detergent fibre, total ash or acid detergent fibre) was included in addition (multiple regression) to D-value obtained with cellulases (Treatment 3). Results of DMD obtained with cellulases show good precision, but underestimate in vivo values, and are closer to those obtained with ruminal fluid. Suitable equations could be used to improve accuracy.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.71 n.2 20112011-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200011en10.4067/S0718-58392011000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	pasture silage digestibility of dry matter
spellingShingle	pasture silage digestibility of dry matter Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
description	In pasture-based dairy herds where silage is a widely adopted supplement, optimized feeding requires reliable estimations of nutritional quality of this conserved forage. Metabolizable energy, an important nutritional fraction, can be predicted from digestibility-related traits, such as the digestible organic matter contained in the dry matter (D-value). The aim of the present study was to evaluate the prediction of D-value and dry matter digestibility (DMD) of grass silages made from four different pastures and maturity stages, using the pepsin-cellulase method. Fungal cellulase was used, applying different enzyme concentrations, incubation times and types of final wash. The silages were prepared from permanent pasture (Dactylis glomerata L., Lolium perenne L., Bromus catharticus Vahl var. catharticus, Trifolium repens L. and Holcus lanatus L.), rotation pasture (Lolium multiflorum Lam. cv. Tama), oats (Avena sativa L.), and mixed pasture (L. perenne-T. repens). These were harvested at three different physiological stages (vegetative, ear emergence and dough grain). The treatment using an incubation time of 24 h, a cellulase concentration of 6.25 g L-1 and final wash with water (Treatment 3) presented the best prediction capacity of the in vivo D-value (R² = 0.78) and in vivo DMD (R² = 0.71). In vivo D-value prediction improved (R² = 0.8) when a chemical determination (crude fibre, gross energy, neutral detergent fibre, total ash or acid detergent fibre) was included in addition (multiple regression) to D-value obtained with cellulases (Treatment 3). Results of DMD obtained with cellulases show good precision, but underestimate in vivo values, and are closer to those obtained with ruminal fluid. Suitable equations could be used to improve accuracy.
author	Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio
author_facet	Barchiesi-Ferrari,Claudia Alomar,Daniel Miranda,Horacio
author_sort	Barchiesi-Ferrari,Claudia
title	Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
title_short	Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
title_full	Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
title_fullStr	Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
title_full_unstemmed	Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages
title_sort	use of cellulases to predict in vivo digestible organic matter (d value) in pasture silages
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200011
work_keys_str_mv	AT barchiesiferrariclaudia useofcellulasestopredictinvivodigestibleorganicmatterdvalueinpasturesilages AT alomardaniel useofcellulasestopredictinvivodigestibleorganicmatterdvalueinpasturesilages AT mirandahoracio useofcellulasestopredictinvivodigestibleorganicmatterdvalueinpasturesilages
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Use of Cellulases to Predict in vivo Digestible Organic Matter (D value) in Pasture Silages

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