In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots
Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this sp...
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| Lenguaje: | English |
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Instituto de Investigaciones Agropecuarias, INIA
2011
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oai:scielo:S0718-583920110003000052018-10-01In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile ShootsGarcía-Gonzáles,RolandoDelgado,MiladysGonzález,YailínGonzález,AníbalGarriga,MiguelCaligari,Peter D.SCarrasco,BasilioQuiroz,Karla Organogenesis in vitro propagation in vitro rooting plant growth regulators Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L-1 6-bencilaminopurine and 3 mg L-1 naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.71 n.3 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300005en10.4067/S0718-58392011000300005 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Organogenesis in vitro propagation in vitro rooting plant growth regulators |
| spellingShingle |
Organogenesis in vitro propagation in vitro rooting plant growth regulators García-Gonzáles,Rolando Delgado,Miladys González,Yailín González,Aníbal Garriga,Miguel Caligari,Peter D.S Carrasco,Basilio Quiroz,Karla In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| description |
Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L-1 6-bencilaminopurine and 3 mg L-1 naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds. |
| author |
García-Gonzáles,Rolando Delgado,Miladys González,Yailín González,Aníbal Garriga,Miguel Caligari,Peter D.S Carrasco,Basilio Quiroz,Karla |
| author_facet |
García-Gonzáles,Rolando Delgado,Miladys González,Yailín González,Aníbal Garriga,Miguel Caligari,Peter D.S Carrasco,Basilio Quiroz,Karla |
| author_sort |
García-Gonzáles,Rolando |
| title |
In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| title_short |
In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| title_full |
In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| title_fullStr |
In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| title_full_unstemmed |
In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots |
| title_sort |
in vitro propagation of cedar (cedrela odorata l.) from juvenile shoots |
| publisher |
Instituto de Investigaciones Agropecuarias, INIA |
| publishDate |
2011 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300005 |
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| _version_ |
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