Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst

Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of...

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Autores principales: Cartes Riquelme,Pricila, Ríos Leal,Darcy, Sáez Carrillo,Kátia, Uribe Moraga,Matilde, Valenzuela Aguilar,Sofía, Joseph Bolus,Stephen, Sánchez Olate,Manuel
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2011
Materias:
Somatic embryogenesis
maturation
germination
HPLC
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007
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spelling oai:scielo:S0718-583920110004000072018-10-01Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) OerstCartes Riquelme,PricilaRíos Leal,DarcySáez Carrillo,KátiaUribe Moraga,MatildeValenzuela Aguilar,SofíaJoseph Bolus,StephenSánchez Olate,Manuel Somatic embryogenesis maturation germination HPLC Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.71 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007en10.4067/S0718-58392011000400007
institution Scielo Chile
collection Scielo Chile
language English
topic Somatic embryogenesis
maturation
germination
HPLC
spellingShingle Somatic embryogenesis
maturation
germination
HPLC
Cartes Riquelme,Pricila
Ríos Leal,Darcy
Sáez Carrillo,Kátia
Uribe Moraga,Matilde
Valenzuela Aguilar,Sofía
Joseph Bolus,Stephen
Sánchez Olate,Manuel
Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
description Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina.
author Cartes Riquelme,Pricila
Ríos Leal,Darcy
Sáez Carrillo,Kátia
Uribe Moraga,Matilde
Valenzuela Aguilar,Sofía
Joseph Bolus,Stephen
Sánchez Olate,Manuel
author_facet Cartes Riquelme,Pricila
Ríos Leal,Darcy
Sáez Carrillo,Kátia
Uribe Moraga,Matilde
Valenzuela Aguilar,Sofía
Joseph Bolus,Stephen
Sánchez Olate,Manuel
author_sort Cartes Riquelme,Pricila
title Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
title_short Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
title_full Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
title_fullStr Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
title_full_unstemmed Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst
title_sort endogenous quantification of abscisic acid and indole-3-acetic acid in somatic and zigotic embryos of nothofagus alpina (poepp. & endl.) oerst
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007
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