Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina

Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease of oilseed plants in Northern Iran. Seventy isolates of M. phaseolina were obtained from different hosts, including soybean (Glycine max L.), and sunflower (Helianthus annuus L.) in the northern oilse...

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Autores principales:	Rayatpanah,Siavosh, Nanagulyan,Siranoush G, Alav,Seyed V, Razavi,Mohammad, Ghanbari-Malidarreh,Abbas
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2012
Materias:	Charcoal rot oilseed plants disease index
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100007
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spelling	oai:scielo:S0718-583920120001000072018-10-01Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolinaRayatpanah,SiavoshNanagulyan,Siranoush GAlav,Seyed VRazavi,MohammadGhanbari-Malidarreh,Abbas Charcoal rot oilseed plants disease index Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease of oilseed plants in Northern Iran. Seventy isolates of M. phaseolina were obtained from different hosts, including soybean (Glycine max L.), and sunflower (Helianthus annuus L.) in the northern oilseed planting regions of Iran. RAPD-PCR amplification profiles, by using of six random OPA primers (kit A) showed polymorphisms among the isolates. The primer OPA-13 (5’_ CAGCACCCAC_3’) amplified the genomic DNA of all isolates of M.phaseolina producing 4-12 bands of sizes between 0.25 and 2.3 kb. Unweighted pair-group method with arithmetic mean (UPGMA) analysis classified the isolates into the nine major groups with 64% similarity. Pathogenicity of isolates was evaluated at seedling stage of soybean, sunflower, and maize (Zea mays L.) plants under in vitro conditions. None of the isolates were pathogenic on corn, while all of the isolates infected soybean and sunflower seedlings. Isolates were more virulent on soybean than sunflower. The disease index on sunflower and soybean varied between 19-24 and 27-30 respectively. These results indicated a significant pathogenic and genetic variability within the Iranian isolates of M. phaseolina. Cultivation with crop rotation was probably tended to induce less diversity of the pathogen isolates.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.72 n.1 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100007en10.4067/S0718-58392012000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Charcoal rot oilseed plants disease index
spellingShingle	Charcoal rot oilseed plants disease index Rayatpanah,Siavosh Nanagulyan,Siranoush G Alav,Seyed V Razavi,Mohammad Ghanbari-Malidarreh,Abbas Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
description	Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease of oilseed plants in Northern Iran. Seventy isolates of M. phaseolina were obtained from different hosts, including soybean (Glycine max L.), and sunflower (Helianthus annuus L.) in the northern oilseed planting regions of Iran. RAPD-PCR amplification profiles, by using of six random OPA primers (kit A) showed polymorphisms among the isolates. The primer OPA-13 (5’_ CAGCACCCAC_3’) amplified the genomic DNA of all isolates of M.phaseolina producing 4-12 bands of sizes between 0.25 and 2.3 kb. Unweighted pair-group method with arithmetic mean (UPGMA) analysis classified the isolates into the nine major groups with 64% similarity. Pathogenicity of isolates was evaluated at seedling stage of soybean, sunflower, and maize (Zea mays L.) plants under in vitro conditions. None of the isolates were pathogenic on corn, while all of the isolates infected soybean and sunflower seedlings. Isolates were more virulent on soybean than sunflower. The disease index on sunflower and soybean varied between 19-24 and 27-30 respectively. These results indicated a significant pathogenic and genetic variability within the Iranian isolates of M. phaseolina. Cultivation with crop rotation was probably tended to induce less diversity of the pathogen isolates.
author	Rayatpanah,Siavosh Nanagulyan,Siranoush G Alav,Seyed V Razavi,Mohammad Ghanbari-Malidarreh,Abbas
author_facet	Rayatpanah,Siavosh Nanagulyan,Siranoush G Alav,Seyed V Razavi,Mohammad Ghanbari-Malidarreh,Abbas
author_sort	Rayatpanah,Siavosh
title	Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
title_short	Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
title_full	Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
title_fullStr	Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
title_full_unstemmed	Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina
title_sort	pathogenic and genetic diversity among iranian isolates of macrophomina phaseolina
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100007
work_keys_str_mv	AT rayatpanahsiavosh pathogenicandgeneticdiversityamongiranianisolatesofmacrophominaphaseolina AT nanagulyansiranoushg pathogenicandgeneticdiversityamongiranianisolatesofmacrophominaphaseolina AT alavseyedv pathogenicandgeneticdiversityamongiranianisolatesofmacrophominaphaseolina AT razavimohammad pathogenicandgeneticdiversityamongiranianisolatesofmacrophominaphaseolina AT ghanbarimalidarrehabbas pathogenicandgeneticdiversityamongiranianisolatesofmacrophominaphaseolina
_version_	1714205305555910656

Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina

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