Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)

Dalaca pallens (Blanchard) is a pest with great impact on grassland production in Chile. The objective of this research was to evaluate the use of entomopathogenic nematodes (EPN) against D. pallens larvae. Twenty EPN isolates, collected along Chile, were used for nematode selection. The experimenta...

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Autores principales:	Maldonado,Alexis, Merino,Loreto, France,Andrés
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2012
Materias:	Biological control lethal concentration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200006
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spelling	oai:scielo:S0718-583920120002000062018-10-01Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)Maldonado,AlexisMerino,LoretoFrance,Andrés Biological control lethal concentration Dalaca pallens (Blanchard) is a pest with great impact on grassland production in Chile. The objective of this research was to evaluate the use of entomopathogenic nematodes (EPN) against D. pallens larvae. Twenty EPN isolates, collected along Chile, were used for nematode selection. The experimental unit consisted of five 50-mL plastic vials filled with Nothofagus dombeyi sawdust and inoculated with 40 dauers per vial. The experimental design was a completely randomized with four replicates per treatment. The results showed that isolate QU N3 (Steinernema australe) and QU N13 (Steinernema unicornum) produced the highest mortality, with 100 and 95% respectively and no differences (P < 0.001) between both. Later, these two isolates were used to calculate the lethal concentration (LC50 and LC90). Concentrations of 0, 10, 20, 30, 40, and 50 dauers per 50-mL plastic vials filled with Nothofagus sawdust were evaluated. Then, a single larva of D. pallens was added to each vial and incubated for 13 d. The results showed that LC50 and LC90 were equivalent to 14 and 39 dauers mL-1 for isolate QU N3, while for isolate QUN13 these figures were 14 and 48 dauers mL-1. Consequently, there are native isolates of EPN with the ability to control D. pallens larvae, which require future test under field conditions.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.72 n.2 20122012-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200006en10.4067/S0718-58392012000200006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biological control lethal concentration
spellingShingle	Biological control lethal concentration Maldonado,Alexis Merino,Loreto France,Andrés Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
description	Dalaca pallens (Blanchard) is a pest with great impact on grassland production in Chile. The objective of this research was to evaluate the use of entomopathogenic nematodes (EPN) against D. pallens larvae. Twenty EPN isolates, collected along Chile, were used for nematode selection. The experimental unit consisted of five 50-mL plastic vials filled with Nothofagus dombeyi sawdust and inoculated with 40 dauers per vial. The experimental design was a completely randomized with four replicates per treatment. The results showed that isolate QU N3 (Steinernema australe) and QU N13 (Steinernema unicornum) produced the highest mortality, with 100 and 95% respectively and no differences (P < 0.001) between both. Later, these two isolates were used to calculate the lethal concentration (LC50 and LC90). Concentrations of 0, 10, 20, 30, 40, and 50 dauers per 50-mL plastic vials filled with Nothofagus sawdust were evaluated. Then, a single larva of D. pallens was added to each vial and incubated for 13 d. The results showed that LC50 and LC90 were equivalent to 14 and 39 dauers mL-1 for isolate QU N3, while for isolate QUN13 these figures were 14 and 48 dauers mL-1. Consequently, there are native isolates of EPN with the ability to control D. pallens larvae, which require future test under field conditions.
author	Maldonado,Alexis Merino,Loreto France,Andrés
author_facet	Maldonado,Alexis Merino,Loreto France,Andrés
author_sort	Maldonado,Alexis
title	Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
title_short	Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
title_full	Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
title_fullStr	Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
title_full_unstemmed	Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)
title_sort	selection of entomopathogenic nematodes against dalaca pallens (lepidoptera: hepialidae)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200006
work_keys_str_mv	AT maldonadoalexis selectionofentomopathogenicnematodesagainstdalacapallenslepidopterahepialidae AT merinoloreto selectionofentomopathogenicnematodesagainstdalacapallenslepidopterahepialidae AT franceandres selectionofentomopathogenicnematodesagainstdalacapallenslepidopterahepialidae
_version_	1714205309758603264

Selection of Entomopathogenic Nematodes against Dalaca pallens (Lepidoptera: Hepialidae)

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