Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes

Botrytis cinerea Pers., the causal agent of gray mold, infects more than 200 plant species. This pathogen has traditionally been controlled by fungicides. However, with the increasing demand for pesticide-free foods new control strategies are needed. The objective of this study was to isolate and se...

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Autores principales:	Vargas,Marisol, Garrido,Felipe, Zapata,Nelson, Tapia,Maritza
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2012
Materias:	Gray mold antagonistic yeast biocontrol agents Vitis vinifera
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300005
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spelling	oai:scielo:S0718-583920120003000052018-10-01Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table GrapesVargas,MarisolGarrido,FelipeZapata,NelsonTapia,Maritza Gray mold antagonistic yeast biocontrol agents Vitis vinifera Botrytis cinerea Pers., the causal agent of gray mold, infects more than 200 plant species. This pathogen has traditionally been controlled by fungicides. However, with the increasing demand for pesticide-free foods new control strategies are needed. The objective of this study was to isolate and select grapevine (Vitis vinifera L.) epiphytic yeasts for the biocontrol of B. cinerea in table grapes. Of the total isolated yeasts (n = 256), 32 exhibited mycelial growth inhibition in dual cultures with a halo > 4 mm, and eight of these isolates inhibited > 90% of conidial germination. When evaluating increasing concentrations on conidial germination inhibition, a dose-dependent response was observed with EC90 values from 0.45 x 10(5) to 0.22 x 10(8) cells mL-1. The antagonistic activity of six yeasts against B. cinerea in table grape berries ’Flame Seedless’ increased as the yeast colonization time increased from 1 to 24 h on the berries, resulting in a higher biocontrol activity on B. cinerea. These results show the effectiveness of grapevine epiphytic yeasts as biocontrol agents of B. cinerea on table grapes.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.72 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300005en10.4067/S0718-58392012000300005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gray mold antagonistic yeast biocontrol agents Vitis vinifera
spellingShingle	Gray mold antagonistic yeast biocontrol agents Vitis vinifera Vargas,Marisol Garrido,Felipe Zapata,Nelson Tapia,Maritza Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
description	Botrytis cinerea Pers., the causal agent of gray mold, infects more than 200 plant species. This pathogen has traditionally been controlled by fungicides. However, with the increasing demand for pesticide-free foods new control strategies are needed. The objective of this study was to isolate and select grapevine (Vitis vinifera L.) epiphytic yeasts for the biocontrol of B. cinerea in table grapes. Of the total isolated yeasts (n = 256), 32 exhibited mycelial growth inhibition in dual cultures with a halo > 4 mm, and eight of these isolates inhibited > 90% of conidial germination. When evaluating increasing concentrations on conidial germination inhibition, a dose-dependent response was observed with EC90 values from 0.45 x 10(5) to 0.22 x 10(8) cells mL-1. The antagonistic activity of six yeasts against B. cinerea in table grape berries ’Flame Seedless’ increased as the yeast colonization time increased from 1 to 24 h on the berries, resulting in a higher biocontrol activity on B. cinerea. These results show the effectiveness of grapevine epiphytic yeasts as biocontrol agents of B. cinerea on table grapes.
author	Vargas,Marisol Garrido,Felipe Zapata,Nelson Tapia,Maritza
author_facet	Vargas,Marisol Garrido,Felipe Zapata,Nelson Tapia,Maritza
author_sort	Vargas,Marisol
title	Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
title_short	Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
title_full	Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
title_fullStr	Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
title_full_unstemmed	Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes
title_sort	isolation and selection of epiphytic yeast for biocontrol of botrytis cinerea pers. on table grapes
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300005
work_keys_str_mv	AT vargasmarisol isolationandselectionofepiphyticyeastforbiocontrolofbotrytiscinereapersontablegrapes AT garridofelipe isolationandselectionofepiphyticyeastforbiocontrolofbotrytiscinereapersontablegrapes AT zapatanelson isolationandselectionofepiphyticyeastforbiocontrolofbotrytiscinereapersontablegrapes AT tapiamaritza isolationandselectionofepiphyticyeastforbiocontrolofbotrytiscinereapersontablegrapes
_version_	1714205312978780160

Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes

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