Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)

The search for alternative methods aiming to the control of agricultural pests that cause less environmental impact has been a recurring theme in several studies. For this purpose, the factors influencing the extraction and isolation of the trypsin inhibitor found in castor-bean (Ricinus communis L....

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Autores principales:	Ramos,Vinicius, Alves,Dejane, Braga,Mariana, Carvalho,Geraldo, Santos,Custódio
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2013
Materias:	Zea mays fall armyworm Ricinus communis trypsin inhibitor alternative control
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200007
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spelling	oai:scielo:S0718-583920130002000072018-10-01Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)Ramos,ViniciusAlves,DejaneBraga,MarianaCarvalho,GeraldoSantos,Custódio Zea mays fall armyworm Ricinus communis trypsin inhibitor alternative control The search for alternative methods aiming to the control of agricultural pests that cause less environmental impact has been a recurring theme in several studies. For this purpose, the factors influencing the extraction and isolation of the trypsin inhibitor found in castor-bean (Ricinus communis L.) cake and seeds were studied, and its effect on the development of fall armyworm Spodoptera frugiperda (Smith, 1797) was evaluated. Therefore, the type and proportion of the solvent (w/v), extraction period and oil content were evaluated. The castor-bean cake extract with oil content of 20% in water at a ratio 1:40 (w/v) for 30 min showed the greatest trypsin inhibition, with 21.23 mUTI g-1 seed. The isolation of the trypsin inhibitor contained in the extract was performed by flash chromatography, with inhibition peaks of up to 8906.12 mUTI g-1 fraction, as well as inferring that the best eluent was ethyl acetate with a small percentage of ethanol (< 30%). In order to evaluate the effect of the inhibitor on the development of this insect, the aqueous extract of castor bean cake with oil content of 20% was incorporated into the diet that was provided to the caterpillars at a concentration of 4000 ppm. There was reduction in the oviposition period and an increase in the concentration of proteins in the feces, with 20.55 ± 3.73 d and 177.70 ± 16.90 μmol g-1, respectively. The increase in protein excretion indicates that the trypsin inhibitor was able to reduce the absorption of essential amino acid residues by the insect without, however, impairing the development of S. frugiperda.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.73 n.2 20132013-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200007en10.4067/S0718-58392013000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Zea mays fall armyworm Ricinus communis trypsin inhibitor alternative control
spellingShingle	Zea mays fall armyworm Ricinus communis trypsin inhibitor alternative control Ramos,Vinicius Alves,Dejane Braga,Mariana Carvalho,Geraldo Santos,Custódio Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
description	The search for alternative methods aiming to the control of agricultural pests that cause less environmental impact has been a recurring theme in several studies. For this purpose, the factors influencing the extraction and isolation of the trypsin inhibitor found in castor-bean (Ricinus communis L.) cake and seeds were studied, and its effect on the development of fall armyworm Spodoptera frugiperda (Smith, 1797) was evaluated. Therefore, the type and proportion of the solvent (w/v), extraction period and oil content were evaluated. The castor-bean cake extract with oil content of 20% in water at a ratio 1:40 (w/v) for 30 min showed the greatest trypsin inhibition, with 21.23 mUTI g-1 seed. The isolation of the trypsin inhibitor contained in the extract was performed by flash chromatography, with inhibition peaks of up to 8906.12 mUTI g-1 fraction, as well as inferring that the best eluent was ethyl acetate with a small percentage of ethanol (< 30%). In order to evaluate the effect of the inhibitor on the development of this insect, the aqueous extract of castor bean cake with oil content of 20% was incorporated into the diet that was provided to the caterpillars at a concentration of 4000 ppm. There was reduction in the oviposition period and an increase in the concentration of proteins in the feces, with 20.55 ± 3.73 d and 177.70 ± 16.90 μmol g-1, respectively. The increase in protein excretion indicates that the trypsin inhibitor was able to reduce the absorption of essential amino acid residues by the insect without, however, impairing the development of S. frugiperda.
author	Ramos,Vinicius Alves,Dejane Braga,Mariana Carvalho,Geraldo Santos,Custódio
author_facet	Ramos,Vinicius Alves,Dejane Braga,Mariana Carvalho,Geraldo Santos,Custódio
author_sort	Ramos,Vinicius
title	Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
title_short	Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
title_full	Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
title_fullStr	Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
title_full_unstemmed	Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
title_sort	extraction and isolation of anti-tryptic castor-bean (ricinus communis l.) substances and their effects on spodoptera frugiperda (smith, 1797) (lepidoptera: noctuidae)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200007
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Extraction and isolation of anti-tryptic castor-bean (Ricinus communis L.) substances and their effects on Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)

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