Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle

Insufficient glycogen content in bovine muscle at slaughter produces meat with high final pH (> 5.8) which is undesirable. The objective of this study was to determine through biopsies the live changes in muscle glycogen concentration (MGC) of housed steers fed on hay or hay plus energy conce...

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Autores principales:	Apaoblaza,Ariel, Gallo,Carmen
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	Cattle muscle biopsies muscle glycogen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000100009
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spelling	oai:scielo:S0718-583920140001000092018-10-01Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscleApaoblaza,ArielGallo,Carmen Cattle muscle biopsies muscle glycogen Insufficient glycogen content in bovine muscle at slaughter produces meat with high final pH (> 5.8) which is undesirable. The objective of this study was to determine through biopsies the live changes in muscle glycogen concentration (MGC) of housed steers fed on hay or hay plus energy concentrate for 30 d and then determine the effect of food deprivation (fasting) for 24 h on the same variable. Ten steers of similar age, weight, and phenotypic characteristics were housed in individual pens and randomly assigned to two feeding treatments: ad libitum hay only (H, n = 5) and ad libitum hay plus flaked corn (Zea mays L.) (HC, n = 5). Biopsies (B) were taken from the Longissimus dorsi muscle at four occasions: the day before the start of experiment (B0), after 15 d (Bl), after 30 d (B2), and fasted for 24 h after B2 (B3). Before each biopsy, steers were sedated with xylazine (0.03 mL kg-1) and lidocaine was applied locally; samples were frozen in liquid nitrogen to determine MGC. Results showed a significant (P < 0.05) effect of diet treatment (HC > H) and also of time (B2 > Bl) on MGC; the decrease in MGC of steers due to fasting (B2 vs. B3) was not significant (P > 0.05). It was concluded that muscle biopsies allowed to detect a difference in the increase of MGC in steers fed an energy supplementation compared to steers fed hay only, and that fasting for 24 h tended to reduce MGC in both groups.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.1 20142014-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000100009en10.4067/S0718-58392014000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cattle muscle biopsies muscle glycogen
spellingShingle	Cattle muscle biopsies muscle glycogen Apaoblaza,Ariel Gallo,Carmen Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
description	Insufficient glycogen content in bovine muscle at slaughter produces meat with high final pH (> 5.8) which is undesirable. The objective of this study was to determine through biopsies the live changes in muscle glycogen concentration (MGC) of housed steers fed on hay or hay plus energy concentrate for 30 d and then determine the effect of food deprivation (fasting) for 24 h on the same variable. Ten steers of similar age, weight, and phenotypic characteristics were housed in individual pens and randomly assigned to two feeding treatments: ad libitum hay only (H, n = 5) and ad libitum hay plus flaked corn (Zea mays L.) (HC, n = 5). Biopsies (B) were taken from the Longissimus dorsi muscle at four occasions: the day before the start of experiment (B0), after 15 d (Bl), after 30 d (B2), and fasted for 24 h after B2 (B3). Before each biopsy, steers were sedated with xylazine (0.03 mL kg-1) and lidocaine was applied locally; samples were frozen in liquid nitrogen to determine MGC. Results showed a significant (P < 0.05) effect of diet treatment (HC > H) and also of time (B2 > Bl) on MGC; the decrease in MGC of steers due to fasting (B2 vs. B3) was not significant (P > 0.05). It was concluded that muscle biopsies allowed to detect a difference in the increase of MGC in steers fed an energy supplementation compared to steers fed hay only, and that fasting for 24 h tended to reduce MGC in both groups.
author	Apaoblaza,Ariel Gallo,Carmen
author_facet	Apaoblaza,Ariel Gallo,Carmen
author_sort	Apaoblaza,Ariel
title	Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
title_short	Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
title_full	Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
title_fullStr	Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
title_full_unstemmed	Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle
title_sort	live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the longissimus dorsi muscle
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000100009
work_keys_str_mv	AT apaoblazaariel livechangesinmuscleglycogenconcentrationofsteersduetofeedingandfastingasdeterminedthroughserialbiopsiesofthelongissimusdorsimuscle AT gallocarmen livechangesinmuscleglycogenconcentrationofsteersduetofeedingandfastingasdeterminedthroughserialbiopsiesofthelongissimusdorsimuscle
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Live changes in muscle glycogen concentration of steers due to feeding and fasting as determined through serial biopsies of the Longissimus dorsi muscle

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