Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance

Rain-induced cracking before harvest is the major cause of crop loss in sweet cherry (Prunus avium [L.] L.) In order to better understand the relationship between cherry fruit cracking and gene expression, the transcriptional patterns of six genes related to cell wall modification and cuticular wax...

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Autores principales:	Balbontín,Cristián, Ayala,Héctor, Rubilar,Joselyn, Cote,Jessica, Figueroa,Carlos R
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	Cell wall and cuticle modification cracking index gene expression analysis Prunus avium
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200006
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spelling	oai:scielo:S0718-583920140002000062018-10-01Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking toleranceBalbontín,CristiánAyala,HéctorRubilar,JoselynCote,JessicaFigueroa,Carlos R Cell wall and cuticle modification cracking index gene expression analysis Prunus avium Rain-induced cracking before harvest is the major cause of crop loss in sweet cherry (Prunus avium [L.] L.) In order to better understand the relationship between cherry fruit cracking and gene expression, the transcriptional patterns of six genes related to cell wall modification and cuticular wax biosynthesis were analyzed during fruit setting (FS), fruit color change (FC) and fruit ripening (FR), employing two contrasting cultivars: the cracking resistant ’Kordia’ and the cracking susceptible ’Bing’. The transcription levels of AP2/EREBP-type transcription factor (PaWINB), wax synthase (WS), ß-ketoacyl-CoA synthase (PaKCS6), and ß-galactosidase (ß-Gal) showed higher levels in ’Kordia’ than in ’Bing’ during the FS stage, while similar values were observed in both cultivars at FR stage. In contrast to that pattern, transcription levels of expansin (PaEXPl) were higher at FR stage in ’Kordia’ than in ’Bing’. Transcript profile of lipid transport protein gene (PaLTPGl) decreased during fruit development, with higher levels in ’Bing’ than in ’Kordia’ at FC and FR stages suggesting no relation with cracking tolerance. The expression profiles of PaWINB, WS, PaKCS6, and ß-Gal suggest that they are genes involved in conferring cracking tolerance, likely due to their function in cuticle deposition during early stages of fruit development. In addition, a greater expression level of expansin gene would allow for a faster growth rate in ’Kordia’ at FR stage.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.2 20142014-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200006en10.4067/S0718-58392014000200006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cell wall and cuticle modification cracking index gene expression analysis Prunus avium
spellingShingle	Cell wall and cuticle modification cracking index gene expression analysis Prunus avium Balbontín,Cristián Ayala,Héctor Rubilar,Joselyn Cote,Jessica Figueroa,Carlos R Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
description	Rain-induced cracking before harvest is the major cause of crop loss in sweet cherry (Prunus avium [L.] L.) In order to better understand the relationship between cherry fruit cracking and gene expression, the transcriptional patterns of six genes related to cell wall modification and cuticular wax biosynthesis were analyzed during fruit setting (FS), fruit color change (FC) and fruit ripening (FR), employing two contrasting cultivars: the cracking resistant ’Kordia’ and the cracking susceptible ’Bing’. The transcription levels of AP2/EREBP-type transcription factor (PaWINB), wax synthase (WS), ß-ketoacyl-CoA synthase (PaKCS6), and ß-galactosidase (ß-Gal) showed higher levels in ’Kordia’ than in ’Bing’ during the FS stage, while similar values were observed in both cultivars at FR stage. In contrast to that pattern, transcription levels of expansin (PaEXPl) were higher at FR stage in ’Kordia’ than in ’Bing’. Transcript profile of lipid transport protein gene (PaLTPGl) decreased during fruit development, with higher levels in ’Bing’ than in ’Kordia’ at FC and FR stages suggesting no relation with cracking tolerance. The expression profiles of PaWINB, WS, PaKCS6, and ß-Gal suggest that they are genes involved in conferring cracking tolerance, likely due to their function in cuticle deposition during early stages of fruit development. In addition, a greater expression level of expansin gene would allow for a faster growth rate in ’Kordia’ at FR stage.
author	Balbontín,Cristián Ayala,Héctor Rubilar,Joselyn Cote,Jessica Figueroa,Carlos R
author_facet	Balbontín,Cristián Ayala,Héctor Rubilar,Joselyn Cote,Jessica Figueroa,Carlos R
author_sort	Balbontín,Cristián
title	Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
title_short	Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
title_full	Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
title_fullStr	Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
title_full_unstemmed	Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
title_sort	transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200006
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Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance

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