Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid

The tobacco aphid Myzus persicae nicotianae Blackman is a subspecies of the highly polyphagous and agricultural pest Myzus persicae (Sulzer). For its control, insecticide applications are widely used, but resistance to numerous molecules has been reported, displaying at least three insecticide resis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cabrera-Brandt,Marco, Silva,Andrea X, Le Trionnaire,Gaël, Tagu,Denis, Figueroa,Christian C
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	Insecticide resistance MACE mechanism Myzus persicae transcriptomic analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392014000200010
record_format	dspace
spelling	oai:scielo:S0718-583920140002000102018-10-01Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphidCabrera-Brandt,MarcoSilva,Andrea XLe Trionnaire,GaëlTagu,DenisFigueroa,Christian C Insecticide resistance MACE mechanism Myzus persicae transcriptomic analysis The tobacco aphid Myzus persicae nicotianae Blackman is a subspecies of the highly polyphagous and agricultural pest Myzus persicae (Sulzer). For its control, insecticide applications are widely used, but resistance to numerous molecules has been reported, displaying at least three insecticide resistance mechanisms, including: (i) elevated carboxylesterases (E-Carb), (ii) modification of the acetylcholinesterase (MACE), and (iii) kdr and super-kdr insensitivity mutations. In Chile, populations of the tobacco aphid are characterized by the presence of a single predominant clone, which is also present in high proportions in other countries of the Americas. This aphid clone exhibits low levels of carboxylesterase activity and is kdr susceptible, but the MACE mechanism of insecticide resistance has not been studied. In order to characterize the tobacco aphid in terms of the MACE mechanism and to identify a preliminary group of aphid genes putatively involved in insecticide resistance, a cDNA microarray was used to study the transcriptomic responses when aphids are sprayed with a carbamate insecticide. The single Chilean clone of the tobacco aphid was characterized as MACE susceptible, but we found 38 transcripts significantly regulated by insecticide exposure (13 up- and 25 down-regulated genes). The expression of six of them was validated by qRT-PCR experiments at several time points (6, 12, 18, 24, 30, 36, and 42 h) after insecticide application. This mutational and transcriptomic characterization of the tobacco aphid responding to insecticide spray opens new hypotheses in the understanding of the molecular mechanisms underlying insecticide resistance.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.2 20142014-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200010en10.4067/S0718-58392014000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Insecticide resistance MACE mechanism Myzus persicae transcriptomic analysis
spellingShingle	Insecticide resistance MACE mechanism Myzus persicae transcriptomic analysis Cabrera-Brandt,Marco Silva,Andrea X Le Trionnaire,Gaël Tagu,Denis Figueroa,Christian C Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
description	The tobacco aphid Myzus persicae nicotianae Blackman is a subspecies of the highly polyphagous and agricultural pest Myzus persicae (Sulzer). For its control, insecticide applications are widely used, but resistance to numerous molecules has been reported, displaying at least three insecticide resistance mechanisms, including: (i) elevated carboxylesterases (E-Carb), (ii) modification of the acetylcholinesterase (MACE), and (iii) kdr and super-kdr insensitivity mutations. In Chile, populations of the tobacco aphid are characterized by the presence of a single predominant clone, which is also present in high proportions in other countries of the Americas. This aphid clone exhibits low levels of carboxylesterase activity and is kdr susceptible, but the MACE mechanism of insecticide resistance has not been studied. In order to characterize the tobacco aphid in terms of the MACE mechanism and to identify a preliminary group of aphid genes putatively involved in insecticide resistance, a cDNA microarray was used to study the transcriptomic responses when aphids are sprayed with a carbamate insecticide. The single Chilean clone of the tobacco aphid was characterized as MACE susceptible, but we found 38 transcripts significantly regulated by insecticide exposure (13 up- and 25 down-regulated genes). The expression of six of them was validated by qRT-PCR experiments at several time points (6, 12, 18, 24, 30, 36, and 42 h) after insecticide application. This mutational and transcriptomic characterization of the tobacco aphid responding to insecticide spray opens new hypotheses in the understanding of the molecular mechanisms underlying insecticide resistance.
author	Cabrera-Brandt,Marco Silva,Andrea X Le Trionnaire,Gaël Tagu,Denis Figueroa,Christian C
author_facet	Cabrera-Brandt,Marco Silva,Andrea X Le Trionnaire,Gaël Tagu,Denis Figueroa,Christian C
author_sort	Cabrera-Brandt,Marco
title	Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
title_short	Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
title_full	Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
title_fullStr	Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
title_full_unstemmed	Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid
title_sort	transcriptomic responses of the aphid myzus persicae nicotianae blackman (hemiptera: aphididae) to insecticides: analyses in the single chilean clone of the tobacco aphid
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000200010
work_keys_str_mv	AT cabrerabrandtmarco transcriptomicresponsesoftheaphidmyzuspersicaenicotianaeblackmanhemipteraaphididaetoinsecticidesanalysesinthesinglechileancloneofthetobaccoaphid AT silvaandreax transcriptomicresponsesoftheaphidmyzuspersicaenicotianaeblackmanhemipteraaphididaetoinsecticidesanalysesinthesinglechileancloneofthetobaccoaphid AT letrionnairegael transcriptomicresponsesoftheaphidmyzuspersicaenicotianaeblackmanhemipteraaphididaetoinsecticidesanalysesinthesinglechileancloneofthetobaccoaphid AT tagudenis transcriptomicresponsesoftheaphidmyzuspersicaenicotianaeblackmanhemipteraaphididaetoinsecticidesanalysesinthesinglechileancloneofthetobaccoaphid AT figueroachristianc transcriptomicresponsesoftheaphidmyzuspersicaenicotianaeblackmanhemipteraaphididaetoinsecticidesanalysesinthesinglechileancloneofthetobaccoaphid
_version_	1714205334888775680

Transcriptomic responses of the aphid Myzus persicae nicotianae Blackman (Hemiptera: Aphididae) to insecticides: Analyses in the single Chilean clone of the tobacco aphid

Ejemplares similares