Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King’s medium B supplemented with 8-hydro...

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Autores principales:	Subramanian,Jayamohan, Satyan,Kumudini
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	IAA PGPR plant growth promotion rhizosphere siderophore
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000300010
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spelling	oai:scielo:S0718-583920140003000102018-10-01Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotypingSubramanian,JayamohanSatyan,Kumudini IAA PGPR plant growth promotion rhizosphere siderophore Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King’s medium B supplemented with 8-hydroxyquinoline (8-HQ) chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA), siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755). Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.3 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000300010en10.4067/S0718-58392014000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	IAA PGPR plant growth promotion rhizosphere siderophore
spellingShingle	IAA PGPR plant growth promotion rhizosphere siderophore Subramanian,Jayamohan Satyan,Kumudini Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
description	Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King’s medium B supplemented with 8-hydroxyquinoline (8-HQ) chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA), siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755). Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.
author	Subramanian,Jayamohan Satyan,Kumudini
author_facet	Subramanian,Jayamohan Satyan,Kumudini
author_sort	Subramanian,Jayamohan
title	Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
title_short	Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
title_full	Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
title_fullStr	Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
title_full_unstemmed	Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
title_sort	isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000300010
work_keys_str_mv	AT subramanianjayamohan isolationandselectionoffluorescentpseudomonadsbasedonmultipleplantgrowthpromotiontraitsandsiderotyping AT satyankumudini isolationandselectionoffluorescentpseudomonadsbasedonmultipleplantgrowthpromotiontraitsandsiderotyping
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Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

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