Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila

Three Candida oleophila strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum on postharvest ’Golden Delicious’ apples (Malus domestica Borkh.) in Chihuahua, Mexico. The in vivo and in vitro activity of exo-...

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Autores principales:	Guerrero,Víctor, Guigon,Cesar, Berlanga,David, Ojeda,Damaris
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	Biocontrol Malus domestica yeasts
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400008
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spelling	oai:scielo:S0718-583920140004000082018-10-01Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophilaGuerrero,VíctorGuigon,CesarBerlanga,DavidOjeda,Damaris Biocontrol Malus domestica yeasts Three Candida oleophila strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum on postharvest ’Golden Delicious’ apples (Malus domestica Borkh.) in Chihuahua, Mexico. The in vivo and in vitro activity of exo-β-1,3-glucanase was measured as a possible biocontrol mode of action for C. oleophila. Mean disease incidence caused by P. expansum was 0.3% for apples treated with fludioxonil + ciprodinil, which were used as a positive control, and 1% for fruits treated with a combination of the three C. oleophila strains; the effects of these treatments were significantly equivalent. Disease incidence in control apples was 39% and was significantly different from the other treatments. The in vivo exo-β-1,3-glucanase activity began at 24 h and peaked at 72 and 96 h for all treatments. Strain L06 had the highest activity (7.96 nKat) and a specific activity of 2.92 nKat μg-1. Candida albicans had the lowest activity (2.83 nKat) and a specific activity of 0.67 nKat ug-1. The highest in vitro activity was for C. albicans (85.03 nKat) and the lowest for strain L06 (78.2 nKat). Significant differences in both in vivo and in vitro enzymatic activity were observed between strain L06 and C. albicans. Polynomial regression analysis (R² = 0.96 in vitro and 0.68 in vivo) indicated that increased enzymatic activity was associated with reduced fruit disease incidence. The production of exo-β-1,3-glucanase by C. oleophila is a possible mode of action for the efficient biocontrol of P. expansum on postharvest apples.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400008en10.4067/S0718-58392014000400008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biocontrol Malus domestica yeasts
spellingShingle	Biocontrol Malus domestica yeasts Guerrero,Víctor Guigon,Cesar Berlanga,David Ojeda,Damaris Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
description	Three Candida oleophila strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum on postharvest ’Golden Delicious’ apples (Malus domestica Borkh.) in Chihuahua, Mexico. The in vivo and in vitro activity of exo-β-1,3-glucanase was measured as a possible biocontrol mode of action for C. oleophila. Mean disease incidence caused by P. expansum was 0.3% for apples treated with fludioxonil + ciprodinil, which were used as a positive control, and 1% for fruits treated with a combination of the three C. oleophila strains; the effects of these treatments were significantly equivalent. Disease incidence in control apples was 39% and was significantly different from the other treatments. The in vivo exo-β-1,3-glucanase activity began at 24 h and peaked at 72 and 96 h for all treatments. Strain L06 had the highest activity (7.96 nKat) and a specific activity of 2.92 nKat μg-1. Candida albicans had the lowest activity (2.83 nKat) and a specific activity of 0.67 nKat ug-1. The highest in vitro activity was for C. albicans (85.03 nKat) and the lowest for strain L06 (78.2 nKat). Significant differences in both in vivo and in vitro enzymatic activity were observed between strain L06 and C. albicans. Polynomial regression analysis (R² = 0.96 in vitro and 0.68 in vivo) indicated that increased enzymatic activity was associated with reduced fruit disease incidence. The production of exo-β-1,3-glucanase by C. oleophila is a possible mode of action for the efficient biocontrol of P. expansum on postharvest apples.
author	Guerrero,Víctor Guigon,Cesar Berlanga,David Ojeda,Damaris
author_facet	Guerrero,Víctor Guigon,Cesar Berlanga,David Ojeda,Damaris
author_sort	Guerrero,Víctor
title	Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
title_short	Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
title_full	Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
title_fullStr	Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
title_full_unstemmed	Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila
title_sort	complete control of penicillium expansum on apple fruit using a combination of antagonistic yeast candida oleophila
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400008
work_keys_str_mv	AT guerrerovictor completecontrolofpenicilliumexpansumonapplefruitusingacombinationofantagonisticyeastcandidaoleophila AT guigoncesar completecontrolofpenicilliumexpansumonapplefruitusingacombinationofantagonisticyeastcandidaoleophila AT berlangadavid completecontrolofpenicilliumexpansumonapplefruitusingacombinationofantagonisticyeastcandidaoleophila AT ojedadamaris completecontrolofpenicilliumexpansumonapplefruitusingacombinationofantagonisticyeastcandidaoleophila
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Complete control of Penicillium expansum on apple fruit using a combination of antagonistic yeast Candida oleophila

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