Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f

Aloe (Aloe vera [L.] Burm. f.) is an important medicinal herb and is propagated vegetatively. However, its propagation rate is slow and cannot supply good quality planting material to large-scale growers. It is therefore necessary to use in vitro clonal propagation for commercial purposes. The aim o...

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Autores principales:	Lavakumaran,Lavanya, Seran,Thayamini H
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2014
Materias:	Aloe BAP microshoots in vitro culture organogenesis TDZ
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400018
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spelling	oai:scielo:S0718-583920140004000182018-10-01Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. fLavakumaran,LavanyaSeran,Thayamini H Aloe BAP microshoots in vitro culture organogenesis TDZ Aloe (Aloe vera [L.] Burm. f.) is an important medicinal herb and is propagated vegetatively. However, its propagation rate is slow and cannot supply good quality planting material to large-scale growers. It is therefore necessary to use in vitro clonal propagation for commercial purposes. The aim of this study was to select a suitable medium for initial culture establishment and subsequent shoot multiplication of aloe. Shoot tips were excised from aloe plants and disinfected with 20% Clorox (5.25% sodium hypochlorite) for 30 min. Sterilized shoot tips were vertically dissected into quarters and then inoculated on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) or thidiazuron (TDZ) (1, 2, and 3 mg L-1) combined with 0.5 mg L-1 1-naphthaleneacetic acid (NAA). Results revealed that there was a significant difference (P < 0.05) between different hormone combinations in shoot bud formation. Explants placed in 3 mg L-1 BAP and 0.5 mg L-1 NAA produced significantly (P < 0.05) higher numbers of microshoots than other tested treatments. The medium containing a low concentration of TDZ (1 mg L-1) with NAA produced multiple microshoots, whereas the highest TDZ concentration (3 mg L-1) with NAA produced high levels of abnormalities. Transferring microshoot buds from 1 mg L-1 TDZ to 3 mg L-1 BAP accelerated production of prominent and elongated shoots. The MS medium supplemented with 3 mg L-1 BAP and 0.5 mg L-1 NAA produced higher number of normal shoots per explant of aloe under in vitro conditions.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.74 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400018en10.4067/S0718-58392014000400018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aloe BAP microshoots in vitro culture organogenesis TDZ
spellingShingle	Aloe BAP microshoots in vitro culture organogenesis TDZ Lavakumaran,Lavanya Seran,Thayamini H Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
description	Aloe (Aloe vera [L.] Burm. f.) is an important medicinal herb and is propagated vegetatively. However, its propagation rate is slow and cannot supply good quality planting material to large-scale growers. It is therefore necessary to use in vitro clonal propagation for commercial purposes. The aim of this study was to select a suitable medium for initial culture establishment and subsequent shoot multiplication of aloe. Shoot tips were excised from aloe plants and disinfected with 20% Clorox (5.25% sodium hypochlorite) for 30 min. Sterilized shoot tips were vertically dissected into quarters and then inoculated on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) or thidiazuron (TDZ) (1, 2, and 3 mg L-1) combined with 0.5 mg L-1 1-naphthaleneacetic acid (NAA). Results revealed that there was a significant difference (P < 0.05) between different hormone combinations in shoot bud formation. Explants placed in 3 mg L-1 BAP and 0.5 mg L-1 NAA produced significantly (P < 0.05) higher numbers of microshoots than other tested treatments. The medium containing a low concentration of TDZ (1 mg L-1) with NAA produced multiple microshoots, whereas the highest TDZ concentration (3 mg L-1) with NAA produced high levels of abnormalities. Transferring microshoot buds from 1 mg L-1 TDZ to 3 mg L-1 BAP accelerated production of prominent and elongated shoots. The MS medium supplemented with 3 mg L-1 BAP and 0.5 mg L-1 NAA produced higher number of normal shoots per explant of aloe under in vitro conditions.
author	Lavakumaran,Lavanya Seran,Thayamini H
author_facet	Lavakumaran,Lavanya Seran,Thayamini H
author_sort	Lavakumaran,Lavanya
title	Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
title_short	Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
title_full	Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
title_fullStr	Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
title_full_unstemmed	Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f
title_sort	effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of aloe vera (l.) burm. f
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400018
work_keys_str_mv	AT lavakumaranlavanya effectof6benzylaminopurineandthidiazurononinvitroshootorganogenesisofaloeveralburmf AT seranthayaminih effectof6benzylaminopurineandthidiazurononinvitroshootorganogenesisofaloeveralburmf
_version_	1714205341846077440

Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f

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