Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)

New cultivars are result of the conservation and characterization of potato (Solanum) genetic resources in secondary germplasm banks. The objectives of this study were to assess phenotype diversity of 12 clones of 10 wild diploid potato species collection super series Rotata, and to determine their...

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Autores principales:	Zeka,Dukagjin, Sedlák,Petr, Sedláková,Vladimíra, Sharma,Kamal, Vejl,Pavel
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	Heterozygozity microsatellite phenotype description PIC
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000200002
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spelling	oai:scielo:S0718-583920150002000022018-10-01Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)Zeka,DukagjinSedlák,PetrSedláková,VladimíraSharma,KamalVejl,Pavel Heterozygozity microsatellite phenotype description PIC New cultivars are result of the conservation and characterization of potato (Solanum) genetic resources in secondary germplasm banks. The objectives of this study were to assess phenotype diversity of 12 clones of 10 wild diploid potato species collection super series Rotata, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Totally 63 alleles of 20 cpSSR loci were detected i.e. 3.15 alleles on average per one microsatellite locus. Alleles ranged from two to six per locus. The highest polymorphism was detected in the locus ntcp9 and lowest were recorded having by two alleles in seven of loci. The average value of observed heterozygosity (Ho) was 0.61, whereas the mean of polymorphic information contents (PIC) was 0.49. Intergenic regions had highest variability (Higr = 0.65) compare with introns (Hin = 0.54) and exons (Hex = 0.45) of the chloroplast genome. Molecular analyses were complemented with tuft morphological measurements according to the descriptor list for the genus Solanum. SSR-based markers highlight a tendency to separate two groups of Rotata wild diploids and show the possibility of duplicities of wild potato genetic resources in the current Czech in vitro collection.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.2 20152015-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000200002en10.4067/S0718-58392015000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Heterozygozity microsatellite phenotype description PIC
spellingShingle	Heterozygozity microsatellite phenotype description PIC Zeka,Dukagjin Sedlák,Petr Sedláková,Vladimíra Sharma,Kamal Vejl,Pavel Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
description	New cultivars are result of the conservation and characterization of potato (Solanum) genetic resources in secondary germplasm banks. The objectives of this study were to assess phenotype diversity of 12 clones of 10 wild diploid potato species collection super series Rotata, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Totally 63 alleles of 20 cpSSR loci were detected i.e. 3.15 alleles on average per one microsatellite locus. Alleles ranged from two to six per locus. The highest polymorphism was detected in the locus ntcp9 and lowest were recorded having by two alleles in seven of loci. The average value of observed heterozygosity (Ho) was 0.61, whereas the mean of polymorphic information contents (PIC) was 0.49. Intergenic regions had highest variability (Higr = 0.65) compare with introns (Hin = 0.54) and exons (Hex = 0.45) of the chloroplast genome. Molecular analyses were complemented with tuft morphological measurements according to the descriptor list for the genus Solanum. SSR-based markers highlight a tendency to separate two groups of Rotata wild diploids and show the possibility of duplicities of wild potato genetic resources in the current Czech in vitro collection.
author	Zeka,Dukagjin Sedlák,Petr Sedláková,Vladimíra Sharma,Kamal Vejl,Pavel
author_facet	Zeka,Dukagjin Sedlák,Petr Sedláková,Vladimíra Sharma,Kamal Vejl,Pavel
author_sort	Zeka,Dukagjin
title	Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
title_short	Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
title_full	Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
title_fullStr	Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
title_full_unstemmed	Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)
title_sort	phenotype and molecular diversity evaluation of some wild 2n solanum species (super series rotata)
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000200002
work_keys_str_mv	AT zekadukagjin phenotypeandmoleculardiversityevaluationofsomewild2nsolanumspeciessuperseriesrotata AT sedlakpetr phenotypeandmoleculardiversityevaluationofsomewild2nsolanumspeciessuperseriesrotata AT sedlakovavladimira phenotypeandmoleculardiversityevaluationofsomewild2nsolanumspeciessuperseriesrotata AT sharmakamal phenotypeandmoleculardiversityevaluationofsomewild2nsolanumspeciessuperseriesrotata AT vejlpavel phenotypeandmoleculardiversityevaluationofsomewild2nsolanumspeciessuperseriesrotata
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Phenotype and molecular diversity evaluation of some wild 2n Solanum species (super series Rotata)

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