Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers

It takes approximately 14 yr to produce a new rice (Oryza sativa L.) variety, that is, from initial hybridization to its commercial release. Currently, new varieties are identified based on morphological descriptors, which have been efficient over time. However, due to the main constraints on seed t...

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Autores principales:	Becerra,Viviana, Paredes,Mario, Gutiérrez,Eduardo, Rojo,Carmen
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	Genetic diversity Oryza sativa SSR variety certification and identification
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400001
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spelling	oai:scielo:S0718-583920150004000012018-10-01Genetic diversity, identification, and certification of Chilean rice varieties using molecular markersBecerra,VivianaParedes,MarioGutiérrez,EduardoRojo,Carmen Genetic diversity Oryza sativa SSR variety certification and identification It takes approximately 14 yr to produce a new rice (Oryza sativa L.) variety, that is, from initial hybridization to its commercial release. Currently, new varieties are identified based on morphological descriptors, which have been efficient over time. However, due to the main constraints on seed type impose to other breeding objectives and the pressure of continuous release of varieties, high degree of parentage, and genetic and morphological uniformity has been observed in the breeding populations. The objectives of this study were: to determine the genetic variability of Chilean and foreign commercial rice varieties, and determine, identify, and certify the genetic relationships among varieties, using simple sequence repeat (SSR) markers. A total of 16 commercial varieties, some of them closely related, were included in the study, which were genétically analyzed using 54 microsatellites. The 54 microsatellite loci allowed the discrimination among the 16 varieties. The number of alleles ranged between 2 and 8 with a mean of 3.54 alleles per locus, while the polymorphism information content (PIC) presented a mean of 0.44. The genetic distance and diversity parameters between pairs of varieties indicate a limited diversity among these genotypes. The cluster analysis indicated that varieties were grouped according to their grain type and pedigree. Results demonstrate that the identification and certification of varieties using microsatellite markers could be a good complement to existing agro-morphological data when varieties are closed related.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400001en10.4067/S0718-58392015000400001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic diversity Oryza sativa SSR variety certification and identification
spellingShingle	Genetic diversity Oryza sativa SSR variety certification and identification Becerra,Viviana Paredes,Mario Gutiérrez,Eduardo Rojo,Carmen Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
description	It takes approximately 14 yr to produce a new rice (Oryza sativa L.) variety, that is, from initial hybridization to its commercial release. Currently, new varieties are identified based on morphological descriptors, which have been efficient over time. However, due to the main constraints on seed type impose to other breeding objectives and the pressure of continuous release of varieties, high degree of parentage, and genetic and morphological uniformity has been observed in the breeding populations. The objectives of this study were: to determine the genetic variability of Chilean and foreign commercial rice varieties, and determine, identify, and certify the genetic relationships among varieties, using simple sequence repeat (SSR) markers. A total of 16 commercial varieties, some of them closely related, were included in the study, which were genétically analyzed using 54 microsatellites. The 54 microsatellite loci allowed the discrimination among the 16 varieties. The number of alleles ranged between 2 and 8 with a mean of 3.54 alleles per locus, while the polymorphism information content (PIC) presented a mean of 0.44. The genetic distance and diversity parameters between pairs of varieties indicate a limited diversity among these genotypes. The cluster analysis indicated that varieties were grouped according to their grain type and pedigree. Results demonstrate that the identification and certification of varieties using microsatellite markers could be a good complement to existing agro-morphological data when varieties are closed related.
author	Becerra,Viviana Paredes,Mario Gutiérrez,Eduardo Rojo,Carmen
author_facet	Becerra,Viviana Paredes,Mario Gutiérrez,Eduardo Rojo,Carmen
author_sort	Becerra,Viviana
title	Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
title_short	Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
title_full	Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
title_fullStr	Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
title_full_unstemmed	Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers
title_sort	genetic diversity, identification, and certification of chilean rice varieties using molecular markers
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400001
work_keys_str_mv	AT becerraviviana geneticdiversityidentificationandcertificationofchileanricevarietiesusingmolecularmarkers AT paredesmario geneticdiversityidentificationandcertificationofchileanricevarietiesusingmolecularmarkers AT gutierrezeduardo geneticdiversityidentificationandcertificationofchileanricevarietiesusingmolecularmarkers AT rojocarmen geneticdiversityidentificationandcertificationofchileanricevarietiesusingmolecularmarkers
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Genetic diversity, identification, and certification of Chilean rice varieties using molecular markers

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