Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage

There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculan...

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Autores principales:	Zielińska,Krystyna, Fabiszewska,Agata, Stefańska,Ilona
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	Aerobic stability alfalfa antibiotic susceptibility Lactobacillus pathogenic bacteria silage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005
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spelling	oai:scielo:S0718-583920150004000052018-10-01Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silageZielińska,KrystynaFabiszewska,AgataStefańska,Ilona Aerobic stability alfalfa antibiotic susceptibility Lactobacillus pathogenic bacteria silage There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005en10.4067/S0718-58392015000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aerobic stability alfalfa antibiotic susceptibility Lactobacillus pathogenic bacteria silage
spellingShingle	Aerobic stability alfalfa antibiotic susceptibility Lactobacillus pathogenic bacteria silage Zielińska,Krystyna Fabiszewska,Agata Stefańska,Ilona Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
description	There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.
author	Zielińska,Krystyna Fabiszewska,Agata Stefańska,Ilona
author_facet	Zielińska,Krystyna Fabiszewska,Agata Stefańska,Ilona
author_sort	Zielińska,Krystyna
title	Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_short	Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_full	Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_fullStr	Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_full_unstemmed	Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_sort	different aspects of lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005
work_keys_str_mv	AT zieli324skakrystyna differentaspectsoflactobacillusinoculantsontheimprovementofqualityandsafetyofalfalfasilage AT fabiszewskaagata differentaspectsoflactobacillusinoculantsontheimprovementofqualityandsafetyofalfalfasilage AT stefa324skailona differentaspectsoflactobacillusinoculantsontheimprovementofqualityandsafetyofalfalfasilage
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Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage

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