Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage

There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculan...

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Autores principales: Zielińska,Krystyna, Fabiszewska,Agata, Stefańska,Ilona
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2015
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005
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spelling oai:scielo:S0718-583920150004000052018-10-01Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silageZielińska,KrystynaFabiszewska,AgataStefańska,Ilona Aerobic stability alfalfa antibiotic susceptibility Lactobacillus pathogenic bacteria silage There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005en10.4067/S0718-58392015000400005
institution Scielo Chile
collection Scielo Chile
language English
topic Aerobic stability
alfalfa
antibiotic susceptibility
Lactobacillus
pathogenic bacteria
silage
spellingShingle Aerobic stability
alfalfa
antibiotic susceptibility
Lactobacillus
pathogenic bacteria
silage
Zielińska,Krystyna
Fabiszewska,Agata
Stefańska,Ilona
Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
description There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.
author Zielińska,Krystyna
Fabiszewska,Agata
Stefańska,Ilona
author_facet Zielińska,Krystyna
Fabiszewska,Agata
Stefańska,Ilona
author_sort Zielińska,Krystyna
title Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_short Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_full Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_fullStr Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_full_unstemmed Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
title_sort different aspects of lactobacillus inoculants on the improvement of quality and safety of alfalfa silage
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005
work_keys_str_mv AT zieli324skakrystyna differentaspectsoflactobacillusinoculantsontheimprovementofqualityandsafetyofalfalfasilage
AT fabiszewskaagata differentaspectsoflactobacillusinoculantsontheimprovementofqualityandsafetyofalfalfasilage
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