Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

Potato Genebank of the Universidad Austral de Chile (UACh) is an important gene bank in Chile. The accessions collected all over the country possess high genetic diversity, present interesting agronomic and cooking traits, and show resistance to biotic and abiotic stress. A particularly interesting...

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Autores principales:	López,Marlon, Riegel,Ricardo, Lizana,Carolina, Behn,Anita
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	Globodera molecular marker Solanum tuberosum PVY PVX
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400008
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spelling	oai:scielo:S0718-583920150004000082018-10-01Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de ChileLópez,MarlonRiegel,RicardoLizana,CarolinaBehn,Anita Globodera molecular marker Solanum tuberosum PVY PVX Potato Genebank of the Universidad Austral de Chile (UACh) is an important gene bank in Chile. The accessions collected all over the country possess high genetic diversity, present interesting agronomic and cooking traits, and show resistance to biotic and abiotic stress. A particularly interesting subgroup of the gene bank includes the accessions collected in the South of Chile, the Chilota Potato Genebank. The focus of this study is the identification of virus and nematode resistant genes in potatoes (Solatium tuberosum L.), using the RYSC3 and YES3-3B molecular markers. The Potato virus Y(PVY) resistance genes Ry adg and Ry sto were identified. Furthermore, the CP60 marker was used to assess the Rx resistance gene that confers resistance to Potato virus X (PVX). In addition, the HC and GRO1-4 markers were utilized to identify the GpaVvrn_QTL and Gro1-4, resistance genes of Globodera pallida and Globodera rostochiensis, respectively. Both G. pallida and G. rostochiensis are Potato Cyst Nematodes (PCN). The plant material used in this study included leaves from 271 accessions of the gene bank. These samples were collected in the field where natural pathogen pressure of potential viruses and diseases exists. ELISA assays were run for field detection of PVY and PVX. However, there have been no previous reports of nematode presence in the plant material. The results herein presented indicate presence of virus and nematode resistance genes in accessions of the Chilota Potato Genebank. In terms of virus resistance, 99 accessions out of the 271 tested possess the Ry adg resistance gene and 17 accessions of these 271 tested have the Ry sto resistance gene. Also, 10 accessions showed positive amplification of the Rxl resistant gene marker. As to nematode resistance, 99 accessions have possible resistance to G. pallida and 54 accessions show potential resistance to G. rostochiensis as detected using the available molecular markers.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400008en10.4067/S0718-58392015000400008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Globodera molecular marker Solanum tuberosum PVY PVX
spellingShingle	Globodera molecular marker Solanum tuberosum PVY PVX López,Marlon Riegel,Ricardo Lizana,Carolina Behn,Anita Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
description	Potato Genebank of the Universidad Austral de Chile (UACh) is an important gene bank in Chile. The accessions collected all over the country possess high genetic diversity, present interesting agronomic and cooking traits, and show resistance to biotic and abiotic stress. A particularly interesting subgroup of the gene bank includes the accessions collected in the South of Chile, the Chilota Potato Genebank. The focus of this study is the identification of virus and nematode resistant genes in potatoes (Solatium tuberosum L.), using the RYSC3 and YES3-3B molecular markers. The Potato virus Y(PVY) resistance genes Ry adg and Ry sto were identified. Furthermore, the CP60 marker was used to assess the Rx resistance gene that confers resistance to Potato virus X (PVX). In addition, the HC and GRO1-4 markers were utilized to identify the GpaVvrn_QTL and Gro1-4, resistance genes of Globodera pallida and Globodera rostochiensis, respectively. Both G. pallida and G. rostochiensis are Potato Cyst Nematodes (PCN). The plant material used in this study included leaves from 271 accessions of the gene bank. These samples were collected in the field where natural pathogen pressure of potential viruses and diseases exists. ELISA assays were run for field detection of PVY and PVX. However, there have been no previous reports of nematode presence in the plant material. The results herein presented indicate presence of virus and nematode resistance genes in accessions of the Chilota Potato Genebank. In terms of virus resistance, 99 accessions out of the 271 tested possess the Ry adg resistance gene and 17 accessions of these 271 tested have the Ry sto resistance gene. Also, 10 accessions showed positive amplification of the Rxl resistant gene marker. As to nematode resistance, 99 accessions have possible resistance to G. pallida and 54 accessions show potential resistance to G. rostochiensis as detected using the available molecular markers.
author	López,Marlon Riegel,Ricardo Lizana,Carolina Behn,Anita
author_facet	López,Marlon Riegel,Ricardo Lizana,Carolina Behn,Anita
author_sort	López,Marlon
title	Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
title_short	Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
title_full	Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
title_fullStr	Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
title_full_unstemmed	Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile
title_sort	identification of virus and nematode resistance genes in the chilota potato genebank of the universidad austral de chile
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400008
work_keys_str_mv	AT lopezmarlon identificationofvirusandnematoderesistancegenesinthechilotapotatogenebankoftheuniversidadaustraldechile AT riegelricardo identificationofvirusandnematoderesistancegenesinthechilotapotatogenebankoftheuniversidadaustraldechile AT lizanacarolina identificationofvirusandnematoderesistancegenesinthechilotapotatogenebankoftheuniversidadaustraldechile AT behnanita identificationofvirusandnematoderesistancegenesinthechilotapotatogenebankoftheuniversidadaustraldechile
_version_	1714205350827130880

Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

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