Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milk-vetch (Astragalus sinicus L., MV) is a major green manure of rice (Oryza sativa L.) fields in southern China, which is recommended as an important agronomic practice to improve s...

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Autores principales:	Fang,Yu, Yan,Zhi-Lei, Chen,Ji-Chen, Wang,Fei, Wang,Ming-Kuang, Lin,Xin-Jian
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	Abundance ammonia-oxidizing archaea (AOA) ammonia-oxidizing bacteria (AOB) Astragalus sinicus community structure Chinese milk vetch soil chemical properties
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500015
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spelling	oai:scielo:S0718-583920150005000152018-10-01Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soilFang,YuYan,Zhi-LeiChen,Ji-ChenWang,FeiWang,Ming-KuangLin,Xin-Jian Abundance ammonia-oxidizing archaea (AOA) ammonia-oxidizing bacteria (AOB) Astragalus sinicus community structure Chinese milk vetch soil chemical properties Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milk-vetch (Astragalus sinicus L., MV) is a major green manure of rice (Oryza sativa L.) fields in southern China, which is recommended as an important agronomic practice to improve soil fertility. Soil chemical properties, abundance and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in a MV-rice rotation field under different fertilization regimes were investigated. The field experiment included six treatments: control, without MV and chemical fertilizer (CK); 100% chemical fertilizer (NPK); 18 000 kg MV ha-1 plus 100% chemical fertilizer (NPKM1); 18 000 kg MV ha-1 plus 40% chemical fertilizer (NPKM2); 18 000 kg MV ha-1 alone (MV); and 18 000 kg MV ha-1 plus 40% chemical fertilizer plus straw (NPKMS). Results showed that NPKMS treatment could improve the soil fertility greatly although the application of 60% chemical fertilizer. The abundance of AOB only in the MV treatment had significant difference with the control; AOA were more abundant than AOB in all corresponding treatments. The NPKMS treatment had the highest AOA abundance (1.19 x 10(8) amoA gene copies g-1) and the lowest abundance was recorded in the CK treatment (3.21 x 10(7) amoA gene copies g-1). The abundance of AOA was significantly positively related to total N, available N, NH4+-N, and NO3--N. The community structure of AOA exhibited little variation among different fertilization regimes, whereas the community structure of AOB was highly responsive. Phylogenetic analysis showed that all AOB sequences were affiliated with Nitrosospira or Nitrosomonas and all AOA denaturing gradient gel electrophoresis (DGGE) bands belonged to the soil and sediment lineage. These findings could be fundamental to improve our understanding of AOB and AOA in the N cycle in the paddy soil.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500015en10.4067/S0718-58392015000500015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Abundance ammonia-oxidizing archaea (AOA) ammonia-oxidizing bacteria (AOB) Astragalus sinicus community structure Chinese milk vetch soil chemical properties
spellingShingle	Abundance ammonia-oxidizing archaea (AOA) ammonia-oxidizing bacteria (AOB) Astragalus sinicus community structure Chinese milk vetch soil chemical properties Fang,Yu Yan,Zhi-Lei Chen,Ji-Chen Wang,Fei Wang,Ming-Kuang Lin,Xin-Jian Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
description	Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milk-vetch (Astragalus sinicus L., MV) is a major green manure of rice (Oryza sativa L.) fields in southern China, which is recommended as an important agronomic practice to improve soil fertility. Soil chemical properties, abundance and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in a MV-rice rotation field under different fertilization regimes were investigated. The field experiment included six treatments: control, without MV and chemical fertilizer (CK); 100% chemical fertilizer (NPK); 18 000 kg MV ha-1 plus 100% chemical fertilizer (NPKM1); 18 000 kg MV ha-1 plus 40% chemical fertilizer (NPKM2); 18 000 kg MV ha-1 alone (MV); and 18 000 kg MV ha-1 plus 40% chemical fertilizer plus straw (NPKMS). Results showed that NPKMS treatment could improve the soil fertility greatly although the application of 60% chemical fertilizer. The abundance of AOB only in the MV treatment had significant difference with the control; AOA were more abundant than AOB in all corresponding treatments. The NPKMS treatment had the highest AOA abundance (1.19 x 10(8) amoA gene copies g-1) and the lowest abundance was recorded in the CK treatment (3.21 x 10(7) amoA gene copies g-1). The abundance of AOA was significantly positively related to total N, available N, NH4+-N, and NO3--N. The community structure of AOA exhibited little variation among different fertilization regimes, whereas the community structure of AOB was highly responsive. Phylogenetic analysis showed that all AOB sequences were affiliated with Nitrosospira or Nitrosomonas and all AOA denaturing gradient gel electrophoresis (DGGE) bands belonged to the soil and sediment lineage. These findings could be fundamental to improve our understanding of AOB and AOA in the N cycle in the paddy soil.
author	Fang,Yu Yan,Zhi-Lei Chen,Ji-Chen Wang,Fei Wang,Ming-Kuang Lin,Xin-Jian
author_facet	Fang,Yu Yan,Zhi-Lei Chen,Ji-Chen Wang,Fei Wang,Ming-Kuang Lin,Xin-Jian
author_sort	Fang,Yu
title	Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
title_short	Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
title_full	Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
title_fullStr	Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
title_full_unstemmed	Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
title_sort	effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500015
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Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

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