Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886) derived from pigeon-pea (Cajanus scarabaeoides (L.) Thouars) accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families de...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mishra,Ramya R, Nag,Atul, Panigrahi,Jogeswar
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2015
Materias:	BSA Cajanus cajan Cajanus scarabaeoides interspecific population linkage mapping marker assisted breeding
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500016
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-58392015000500016
record_format	dspace
spelling	oai:scielo:S0718-583920150005000162018-10-01Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-peaMishra,Ramya RNag,AtulPanigrahi,Jogeswar BSA Cajanus cajan Cajanus scarabaeoides interspecific population linkage mapping marker assisted breeding The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886) derived from pigeon-pea (Cajanus scarabaeoides (L.) Thouars) accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families derived from an interspecific cross C. cajan (L.) Huth (’ICP-26’) x C. scarabaeoides (accession ICPW-94) revealed monogenic gene action for resistance to plume moth, and the dominant control by single locus or cluster of tightly linked alleles. Bulked segregant analysis (BSA) of 116 F2 progenies by using 143 parental polymorphic RAPD primers could identify a fragment OPA09(910) associated with plume moth resistance in coupling phase of linkage. Further single plant analysis of the 116 F2 mapping population revealed OPA09(910) was linked to PPMi locus conferring host resistance to plume moth with recombination fraction (rf) value of 0.125 (12.7 cM of Kosambi function). The resistance specific fragment OPA09(910) was cloned, sequenced and converted into a sequence characterized amplified region (SCAR) marker, SCOPA09(942), which was also closely associated (10.3 cM) with the locus PPMl with rf value 0.102. BLAST analysis with pigeon-pea genome sequence also confirmed its occurrence in CcLG02 (Scafseq.LG_V5.0fa) and contig 01597 (AFSP01.fsa1). This SCAR marker showed reasonable screening efficiency in the F2, F3, and BC1F1 lines, thus it can be used as genetic handle in marker-assisted introgression of the genomic fragment conferring plume moth resistance and screening of breeding lines in pigeon-pea.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.75 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500016en10.4067/S0718-58392015000500016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	BSA Cajanus cajan Cajanus scarabaeoides interspecific population linkage mapping marker assisted breeding
spellingShingle	BSA Cajanus cajan Cajanus scarabaeoides interspecific population linkage mapping marker assisted breeding Mishra,Ramya R Nag,Atul Panigrahi,Jogeswar Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
description	The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886) derived from pigeon-pea (Cajanus scarabaeoides (L.) Thouars) accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families derived from an interspecific cross C. cajan (L.) Huth (’ICP-26’) x C. scarabaeoides (accession ICPW-94) revealed monogenic gene action for resistance to plume moth, and the dominant control by single locus or cluster of tightly linked alleles. Bulked segregant analysis (BSA) of 116 F2 progenies by using 143 parental polymorphic RAPD primers could identify a fragment OPA09(910) associated with plume moth resistance in coupling phase of linkage. Further single plant analysis of the 116 F2 mapping population revealed OPA09(910) was linked to PPMi locus conferring host resistance to plume moth with recombination fraction (rf) value of 0.125 (12.7 cM of Kosambi function). The resistance specific fragment OPA09(910) was cloned, sequenced and converted into a sequence characterized amplified region (SCAR) marker, SCOPA09(942), which was also closely associated (10.3 cM) with the locus PPMl with rf value 0.102. BLAST analysis with pigeon-pea genome sequence also confirmed its occurrence in CcLG02 (Scafseq.LG_V5.0fa) and contig 01597 (AFSP01.fsa1). This SCAR marker showed reasonable screening efficiency in the F2, F3, and BC1F1 lines, thus it can be used as genetic handle in marker-assisted introgression of the genomic fragment conferring plume moth resistance and screening of breeding lines in pigeon-pea.
author	Mishra,Ramya R Nag,Atul Panigrahi,Jogeswar
author_facet	Mishra,Ramya R Nag,Atul Panigrahi,Jogeswar
author_sort	Mishra,Ramya R
title	Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
title_short	Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
title_full	Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
title_fullStr	Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
title_full_unstemmed	Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea
title_sort	development of dominant sequence characterized amplified region (scar) marker linked with plume moth (exelastis atomosa walsingham 1886) resistance in pigeon-pea
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000500016
work_keys_str_mv	AT mishraramyar developmentofdominantsequencecharacterizedamplifiedregionscarmarkerlinkedwithplumemothexelastisatomosawalsingham1886resistanceinpigeonpea AT nagatul developmentofdominantsequencecharacterizedamplifiedregionscarmarkerlinkedwithplumemothexelastisatomosawalsingham1886resistanceinpigeonpea AT panigrahijogeswar developmentofdominantsequencecharacterizedamplifiedregionscarmarkerlinkedwithplumemothexelastisatomosawalsingham1886resistanceinpigeonpea
_version_	1714205354638704640

Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

Ejemplares similares